原发性肝细胞癌中乙型肝炎病毒X抗原表达的分析  

ANALYSIS OF HBV X ANTIGEN EXRESSION IN HEPATOCELLULAR CARCINOMA

作　　者：黄耀煊[1] 邱光惠 吴霞[1] 范公忍[1] 皋岚湘[1] 王锡育 黄洁[1] 邓燕林[1] 

机构地区：[1]北京军区总医院肝病研究所,白求恩国际和平医院传染科,解放军总医院肝胆外科

出　　处：《肝脏病杂志》1995年第2期86-88,共3页

摘　　要：我们前已报道用抗ＨＢＶｘ抗原（ＨＢｘＡｇ）三种不同肽段的抗体检出肝癌细胞中ＨＢｘＡｇ的存在。我们用ＰＣＲ技术扩增肝癌肝组织中的ｘ基因，用重组ＨＢｘＡｇ及其抗体分别检测肝癌患者血清中的抗ＨＢｘ和ＨＢｘＡｇ并用免疫组织化学的方法分析肝组织中ＨＢｘＡｇ的表达及其分布。我们发现ＨＢｘＡｇ在肝癌肝组织中阳性率高达９２．６％而且分布广泛，主要表达在细胞浆，但细胞核内亦有见到。癌旁组织检出率也很高，而且在小胆管上皮细胞内亦见有ＨＢｘＡｇ的存在。In our previous papers intrahepatic localization of HB xAg in liver cancer was investigated usingantibodies against peptide fragments representing three particular regions of X gene sequence. In thisstudy PCR technique was used to detect the X gene in the liver tissue sections.The antisera against therecombinant HBxAg which was synthesized in E. coli were prepared andused for the detection ofHBxAg in the liver cell of 7 8 patients with HCC. And the HBxAg/anti HBx were also analizedin the sera from the patients using ELISA. It was found that HBxAg was not only seen in hepatocyte cyto-plasm but also in nuclei and the total positivity rate of HBxAg is 92.6%(75/78). Besides this, we alsofound the predominant expression of HBXAg in bile duct ethelial cells. Our findings show that theexpression of HBxAg closely related to the development of HCC.

关 键 词：肝细胞癌 乙型肝炎病毒 X抗原 基因表达 

分 类 号：R735.702[医药卫生—肿瘤] R373.21[医药卫生—临床医学]