大鼠纹状体灌流谷氨酸对局部羟自由基生成的影响  

Effect of Perfusing Glutamate on The Local Hydroxyl Radicals in Corpus Striatum of Rats

作　　者：黄新祥[1] 黄诒森[2] 

机构地区：[1]镇江医学院临床生化教研室 [2]镇江医学院基础生化教研室

出　　处：《镇江医学院学报》1995年第4期274-274,276,共2页Journal of Zhenjiang Medical College

摘　　要：采用脑区微灌流系统和水杨酸为捕捉剂的ＨＰＬＣ羟自由基检测法，观察到大鼠纹状体灌流０．１ｍｍｏｌ／Ｌ谷氨酸１５～３０ｍｉｎ内，灌流液中羟自由基和水杨酸反应生成的衍生物２，５－ＤＨＢＡ的浓度为３１０．４±１７１．８ｎｇ／ｍｌ（ｘ±ｓ，ｎ＝５）较谷氨酸灌流前的８４．８±３７．０ｎｇ／ｍｌ（ｘ±ｓ，ｎ＝５）含量显著增高（Ｐ＜０．０１）．而给予氯胺酮０．６ｍｇ／ｍｌ加灌组，谷氨酸灌流１５～３０ｍｉｎ内的２，５－ＤＨＢＡ浓度为１６５．４±８８．１ｎｇ／ｍｌ（ｘ±ｓ，ｎ＝５）。结果提示纹状体细胞外谷氨酸含量增加能使局部羟自由基生成增加且能被氯胺酮部分抑制。Effect of glutamate on hydroxyl radical in rat corpus striatum has been studied.Based on the pashpull microperfusion system and HPLC-UVdetection,we found that the concentration of 2,5-DHBA induced by salicylate capturing hydroxyl radical in the microperfusate was significantly increased during 15￣30 mn of perfusing glutamate(0.1mmol·L-1)to corpus striatum.The increasing concertration of 2,5-DHBA was partly reduced by perfusing glutamate and ketamin (0.6mg·ml-1)simultaneously.The results suggest that increasing extracellular concentration of glutamate can raise the local concentration of hydroxyl radical in rat striatum,and ketamin can depress this effect.

关 键 词：谷氨酸 羟自由基 纹状体 微灌流 缺血性脑损伤 

分 类 号：Q517[生物学—生物化学] R743.31[医药卫生—神经病学与精神病学]