脑缺血再灌注后脑组织C-fos基因表达与丹参的影响  

Effect of Radix Salviae Miltiorrhizae (RSM) on C-fos gene expression in Rat Brain after Cerebral Ischemia and Reperfusion

作　　者：吴卫平[1] 匡培根[1] 李振洲[1] 

机构地区：[1]解放军总医院神经介质实验室,100853

出　　处：《临床神经科学》1995年第2期63-65,共3页Chinese Journal of Clinical Neurosciences

摘　　要：采用线栓法制成大鼠大脑中动脉缺血再灌注模型,用地高辛精标记C-fos探针进行原位杂交。结果示缺血再灌注鼠栓塞侧和丹参组栓塞侧皮层及海马C-fos基因表达显著增多,但丹参组栓塞侧C-fos基因表达显著低于缺血再灌组(P<0.05),而两组栓塞对侧比较无显著差异。表明,脑缺血再灌注后脑组织C-fos基因表达显著增多,丹参能部分抑制缺血后C-fos基因的表达。The local cerebral ischemia and reperfusion model of rats was made with thread embolism of middle cerebral artery. After 90 min ischemia following 30 min reperfusion, C-fos mRNA expression in rat brain was investigated with in situ hybridization in ischemia following reperfusion and RSM-treated group. The results showed that C-fos gene expression in cerebral cortex and hippocampus of ischemic sides was significantly increased as compared with nonischemic sides (P<0. 001). Although C-fos gene expression in RSM treated-rat was also increased, yet it was significantly lower than that of ischemia following reperfusion group (P<0. 05). The results indicated that C-fos gene expression in rat brain can be induced by cerebral ischemia following reperfusion and RSM may partly play a role in inhibition of C-fos gene expression during ischemia and reperfusion.

关 键 词：脑缺血 再灌注 丹参 C-FOS基因 

分 类 号：R743.310.2[医药卫生—神经病学与精神病学] R277.733.1[医药卫生—临床医学]