应激引起大鼠行为改变可能与中枢谷氨酸系统有关  

Glutamate System in CNS Correlated with Changes of Stress-induced Behavior in Rat

作　　者：叶阿莉[1] 王春安 陈宜张 薛明园 胡宗礼[3] 朱铨英 

机构地区：[1]第二军医大学基础部生理学教研室神经科学实验室,200433 [2]上海市长宁区卫生学校基础组 [3]湖北省咸宁医学院药理室 [4]第二军医大学基础部药理学教研室,200433

出　　处：《临床神经科学》1995年第3期133-136,共4页Chinese Journal of Clinical Neurosciences

摘　　要：本实验以旷场试验法测定动物在急、慢性躯体性和心理性应激时的行为变化;以快速杀头冷冻匀浆法,用HITACHI-835氨基酸分析仪测定应激各期视皮层、海马、下丘脑、小脑谷氨酸含量,并经侧脑室微量注射L-AP_4后观察行为变化。结果显示,急性应激动物行为活动增加,慢性应激减少;躯体性和心理性应激时,大鼠四脑区的谷氨酸含量与对照组相比在不同时期呈现显著性差异,躯体性和心理性应激组之间谷氨酸含量有显著性差异的是:视皮层、海马、侧脑室微量注射L-AP_4后可致行为活动减弱。Glutamate (Glu) levels of visual cortex (VC), hippocampus (Hi), hypothalamus (Hy) and cerebellum (Ce) during acute and chronic, and physical and psychological stress in rats were measured. Open-field behaviors (OFBs ) during stress and after intracere- broventricularly (icv, lateral ventricle) administered L-AP4 (10-3mol/L, 5μl) or saline (5μl) were recorded with TV camera. The results showed that Glu levels of Hi and Hy in acute stress group and that of VC in chronic stress groups were higher and that of Ce was lower than that in control (P<0. 05) , and there was a significant difference in Glu level of Hi between physical and psychological stress. OFBs were markedly reduced during chronic stress. It was similar to chronic stressed animals that OFBs in non-stress rats were reduced significantly in the 5 min following.icv administration of L-AP4. These suggested that stress-induced OFBs change was connected with Glu system in CNS.

关 键 词：应激 旷场试验 谷氨酸 L-AP4 

分 类 号：R363[医药卫生—病理学]