水稻籼粳杂种人工种子制备的研究  

Studies on Production of Artificial Seeds of Rice Hybrid between Indica and Japonica

作　　者：邢小黑[1] 尹道川 沈毓渭[2] 高明尉[2] 

机构地区：[1]江苏省农业科学院,江苏南京210014 [2]浙江农业大学,浙江杭州310029

出　　处：《作物学报》1995年第1期45-48,T001,共5页Acta Agronomica Sinica

基　　金：浙江省科委自然科学基金资助

摘　　要：从二个水稻籼粳杂种幼穗来源的愈伤组织上,分化出大量不定芽。在2,4-D0.2mg/L、BAP2mg/L的MS培养基上,亚优2号不定芽增殖率可达3.5。无菌条件下,以不定芽作材料制备的人工种子发芽率最高可达60.0％,最低也可达15.0％。使用麦芽糖代替蔗糖,以及用较大的不定芽都可提高人工种子发芽率。The effects of different factors in artificial seeds of rice hybrid between Indica and Japonica were investigated to establish the basic technology for artificial seeds preparation with improved culture conditions. A large quantity and high frequency of adventitious buds were obtained from calli on MS medium supplemented with 0.2 mg/ L 2,4-D and 2 mg/ L BAP. on which, multiplication coefficient of Yayou 2 hao achieved to 3.5. The germination rate of artificial seeds with encapsulated buds under sterile conditions was from 15.0% to 60.0% on vermiculite and agar bases. To use maltose in place of sucrose and improve quality of adventitious buds could raise germination rate of artificial seeds.

关 键 词：水稻 籼粳杂种 不定芽 人工种子 

分 类 号：S511.203.2[农业科学—作物学]