对三苯氧胺耐药性大鼠乳腺癌细胞株的建立  

THE ESTABLISHMENT OF TAMOXIFEN-RESISTANT RAT MAMMARY TUMOUR CELL STRAIN

作　　者：尹科祥[1] 苏宁[1] 张瑞英[1] 

机构地区：[1]南京铁道医学院病理教研室

出　　处：《癌症》1995年第3期170-175,共6页Chinese Journal of Cancer

摘　　要：三苯氧胺（ＴＡＭＯＸＩＦＥＮ，ＴＸＦ）是临床乳腺癌内分泌治疗中重要的抗雌激素药物之一，长期用药可致耐药性的产生。体内体外实验模型的建立有助于阐明耐药性的机理。本文应用改良的Ｈｕｇ′Ｓ双层琼脂培养法，对ＳＨＺ－８８细胞进行克隆培养，筛选出耐受ＴＸＦ的大鼠乳腺癌细胞株。该细胞株对药物ＴＸＦ的敏感性比原代细胞降低十倍，但对雌激素的反应性无改变。该细胞株的建立将有助于研究耐药乳腺肿瘤细胞的生物学行为，并可用于筛选抗耐药乳腺肿瘤药物。Tamoxifen is a noncytotoxic drug for endocrine-responsive humanbreast cancer. Tumours. which initally respond well to tamoxifen, develop resistance withtime. The establishment of experimental models in vivo and in vitro will be helpful tothe discovery of the tamoxifen-resistant mechanism. Using improved Hug’s double-layersoft agar culture, we have selected a tamoxifen-resistant rat mammary tumour cell strainfrom the SHZ-88 cell line(designatedas Y2 cell).Y2 cell is ten times insensitivity to ta- moxifen as conlphred to the parent cell line, and no sensitive response to estrogens。 Theestablishment of Y2 cell strain provided a practical tool to study the biological charac- terization of drug-resistant mammary tumour cells and select the effective anticancerdrugs for the tamoxifen-resistant mammary tumour.

关 键 词：乳腺癌 三苯氧胺 耐药性 细胞株 大鼠 

分 类 号：R737.905[医药卫生—肿瘤]