人肺癌组织中C-Ha-ras,C-myc和C-sis基因的扩增  

AMPLIFICATION OF C-Ha-ras, C-myc AND C-sis ONCOGENE IN HUMAN LUNG CANCER

作　　者：何玲[1] 詹德进 陈家堃[1] 易菲[1] 吴中亮[1] 杜应秀[1] 

机构地区：[1]广州医学院化学致癌研究所,广州510182

出　　处：《癌症》1995年第4期259-261,共3页Chinese Journal of Cancer

摘　　要：用同位素ａ－３２ＰｄＣＴＰ标记癌基因Ｃ－Ｈａ－ｒａｓ，Ｃ－ｍｙｃ和Ｖ－ｓｉｓ基因片段，通过点杂交和Ｓｏｕｔｈｅｒｎ杂交法检测１７例鳞癌，９例腺癌，６例其它类型肺癌组织和６例非肺癌组织中Ｃ－Ｈａ－ｒａｓ，Ｃ－ｍｙｃ和Ｃ－ｓｉｓ基因扩增的情况．结果发现：４６．８％（１５／３２）的肺癌组织有Ｃ－Ｈａ－ｒａｓ基因扩增，其中１０例（５８．８ ％）鳞癌，３例腺癌（３３．３ ％）出现Ｃ－Ｈａ－ｒａｓ基因扩增。２５％（８／３２）的肺癌组织中出现Ｃ－ｍｙｃ基因扩增，其中 ４例（２３．５％）鳞癌，３例（３３．３％）腺癌出现Ｃ－ｍｙｃ基因扩增。１８．８％（６／３２）的肺癌组织有Ｃ－ｓｉｓ基因扩增，其中４例（２３．５％）鳞癌，１例（１１．１％）腺癌出Ｃ－ｓｉｓ基因扩增。而６例非肺癌组织都役有这三种癌基因扩增。同时我们也发现有５例肺癌组织（４例鳞癌和１例腺癌）出现两种癌基因的扩增。上述结果提示：Ｃ－Ｈａ－ｒａｓ，Ｃ－ｍｙｃ和Ｃ－ｓｉｓ基因的扩增与肺癌的发生有关，特别是Ｃ－Ｈａ－ｒａｓ基因扩增在肺鳞癌的形成过程中可能起着重要作用。同时还提示：肺癌的发生与两种癌基因的扩增可能有一定关系。Anaiysis was performed for detecting amplification of C-Ha-ras, C-myc and C-sis oncogene in 32 samples of lung cancer tissues and 6 sampies of non- lung cancer tissues. The results showed that C-Ha-ras gene amplification was observed in 10 of 17 squamous carcinomas(58.8%), 3 of 9 adenocarcinomas(33.3%) C-myc gene amplitication was observed in 4 of 17 squamous carcinomas(23.5%), 3 of 9(33.3%) ade- nocarcinomas and C-sis gene was observed in 4 of 17 squamous carcinomas(23.5%), 1 of 9(11.1%) adenocarcinomas. The amplification of these three oncogenes was not detected in 6 non lung cancer tissues, But two oncogenes amplified simultaneously in 4 of squam- ous carcinomas and 1 of adenocarcinomas, These results suggested that the amplification of the three oncogenes is correlated with the carcinogenesis of lung cancer. Especially, the amplification of C-Ha-ras gene may play an important role in the development of squamous carcinomas. The amplification of two oncogene might be correlated with the pathogenesis of lung cancer.

关 键 词：癌基因 基因扩增 肺肿瘤 C-HA-RAS C-MYC C-SIS 

分 类 号：R734.202[医药卫生—肿瘤]