光敏生物素标记探针原位杂交检测肝癌基因表达  

DETECTION OF GENE EXPRESSION IN HEPATOCELLULAR CARCINOMA BY IN SITU HYBRIDIZATION WITH PHOTOBIOTIN-LABELLED PROBE

作　　者：杨少波[1] 王孟薇[1] 尤伟缔[1] 陆应麟[1] 陈堃[1] 

机构地区：[1]北京解放军三0四医院消化科,解放军总医院南楼消化科,军事医学科学院基础所

出　　处：《癌症》1995年第5期356-358,共3页Chinese Journal of Cancer

摘　　要：运用光敏生物素标记基因探针对２３例新鲜肝癌和癌旁组织的ｃ－ｍｙｃｍＲＮＡ和ｐ５３ｍＲＮＡ进行原位杂交（ｃＤＮＡ－ｍＲＮＡ）检测，发现在部分组织切片中存在内源件生物素样物质而产生假阳性，本实验采用一种封闭内源性生物素样物质的方法解决了假阳性问题，对这一方法的机理进行了讨论。pairs of fresh tissues of hepatocellular carcinoma(HCC) and corresponding non-tumorous liver(NT) had been studied by situ hybridization(cDNA- mRNA)With photobiotin-labelled probes to detect the expression of c- myc mRNA and p53 mRNA. It was discovered that some cells in some sections showed endogenous avidin-binding activity(EABA)which resulted in pseudo- signals. A method of blocking EABA is introduced and its mechanism is discussed. The results showed that it is a useful method.

关 键 词：生物素 亲和素 原位杂交 基因表达 肝肿瘤 诊断 

分 类 号：R735.704[医药卫生—肿瘤]