K562／Adm多药耐药细胞株生物学特性的进一步研究  

FURTHER IDENTIFICATION OF BIOLOGICAL CHARACTERISTICS OF K562MULTIDRUGRESISTANT TO ADRIAMYCINCEL LINE

作　　者：刘超[1] 黄一微 谢彦晖[1] 谢毅[1] 丁训杰[1] 沈世人 苏颖 单易非[1] 高红阳[1] 

机构地区：[1]上海医科大学华山医院血液科,福建省福州市肿瘤医院研究室,上海医科大学基础部生物物理教研室

出　　处：《癌症》1995年第6期415-417,共3页Chinese Journal of Cancer

基　　金：中国卫生部青年科研基金

摘　　要：采用流式细胞仪及逆转录－多聚酶链式反应技术对所建立的Ｋ５６２／Ａｄｍ细胞株生物学特性进一步研究。结果表明该耐药细胞对化疗药物的摄取能力明显降低，细胞跨膜糖蛋白Ｐ－１７０及编码该蛋白的ｍｄｒ－１ｍＲＮＡ具有过度表达。细胞酶学研究发现耐药细胞Ｇ６ＰＤ及６ＰＧＤ活性明显升高，尤以Ｇ６ＰＤ为著。该细胞为多药耐药性及其相关机制的研究提供一良好模型。Using flow cytometry and reverse transcription polymerase chain reaction(RT PCR), the biological characteristics of K562 multidrug resistant to adriamycine(K562/Ad)cell line were identified. The remarkable findings were the reduced intracellular accumulation ofdaunorubicine(DNR),the overexpression of p glycoprotein(P gp)as well as the mdr 1mRNAwhich encodes P gp in K562/Adm cell line in comparison to its parental sensitive K562 cell line.enxymic analysis showed that G6PD and 6GPD, especially the former elevated significi1ntly. Ourresults indicated that K562/Adm cell line might be used as an appropriate model for the study ofmultidrug resistance and its related mechanisms.

关 键 词：K562细胞 多药耐药性 肿瘤 

分 类 号：R73－361[医药卫生—肿瘤]