rhTNF－α对AML原代白血病细胞c－myc原癌基因表达的影响  

EFFECT ON C-MYC EXPRESSION OF ACUTE MYELOCYTIC LEUKEMIC FRESH CELL BY rhTNF-α

作　　者：袁跃传[1] 沈素芸[1] 

机构地区：[1]解放军第四军医大学西京医院

出　　处：《白血病》1995年第1期13-15,共3页

摘　　要：采用细胞总ＲＮＡ斑点杂交技术，以￣３２Ｐ标记的ｃ－ｍｙｃＤＮＡ片段为探针，研究了８例急性髓细胞白血病（ＡＭＬ）原代白血病细胞ｃ－ｍｙｃ原癌基因的表达及其径小剂量重组人肿瘤坏死因子（ｒｈＴＮＦ－α）处理后ｃ－ｍｙｃｍＲＮＡ水平的变化。结果发现：全部８例病人的骨髓细胞均呈Ｃ－ｍｙｃ高表达，其中６例经５０ｕ／ｍｌＴＮＦ诱导处理１２小时后，其ｃ－ｍｙｃｍＲＮＡ水平发生显著降低，另２例的抑制程度较轻。结果证明ＴＮＦ对ＡＭＬ原代白血病细胞ｃ－ｍｙｃ表达具有明显的抑制作用，从而预示ＴＮＦ在ＡＭＬ诱导分化治疗应用的潜在有效性。The total RNA was prepared to dot blot with ￣32P nick-translated c-myc DNA probe, Westudied the c-myc expression of fresh bone marrow cells in AML patients treated by rhTNF-αin8 cases. The results showed that the expression of c-myc was high in all 8 cases,in which c-mycexpression was inhibited by 50u /ml TNF treated 12 hours la ter in 6 cases,the other 2 casesshowed mimimal changes. The results demonstrated that TNF-α has inhibiting effect on c-mycexpression of fresh leukemic cells in AML, It also indicated the possible effect of inducingdifferentiation in the treatment of AML by low-dose rhTNF-α.

关 键 词：髓细胞 白血病 原癌基因 肿瘤坏死因子 RHTNF-Α 

分 类 号：R733.710.2[医药卫生—肿瘤]