应用PCR技术检测细小病毒H－1DNA在人肝癌与裸鼠正常组织中复制的差异  

A HIGHLY SENSITIVE AND SPECIFIC PCR METHOD FOR DETECTION OF REPLICATION OF PARVOVIRUS H-1 AND MVM IN VITRO AND IN VIVO

作　　者：黄青山[1] 马承武 郭兰萍[1] 陈献华[1] 罗祖玉[1] 

机构地区：[1]上海复旦大学生理与生物物理学系

出　　处：《病毒学报》1995年第1期84-87,共4页Chinese Journal of Virology

基　　金：国家自然科学基金委员会;复旦大学科学基金;国际抗癌联盟(UICC)等资助

摘　　要：应用ＰＣＲ技术检测细小病毒Ｈ－１ＤＮＡ在人肝癌与裸鼠正常组织中复制的差异黄青山，马承武，郭兰萍，陈献华，罗祖玉（上海复旦大学生理与生物物理学系，上海２００４３３）关键词：自主性细小病毒Ｈ－１及ＭＶＭ，聚合酶链式反应（ＰＣＲ），人肝癌模型，抑瘤作用，肿...highly sensitive and specific PCR method for the detection of the autonomousParvoviruses H-1 (H-1) and minute virus of mice (MVM) was established by the constructionof two primers, P3 and P4, copying sequences encoding the non-structural (NS) proteins ofH-1 and MVM. The method had been used successfully for monitoring H-1 replicationboth in in vitro system, NB-K cells and human hepatonia cells, QGY-7703, and in thetu-mor-bearing nude mice. Several pfu of H-1 could be detected in vitro by a specific ampli-fied H-1 DNA band (908bp), and 6 hrs after H-1 infection of NB-K cells. The virus spe-cific DNA band was detected in DNA extracted from ca 250 infected cell. H-1 (1×10￣8pfu)inoculation of the tumor-bearing nude miee was accmplished by intravenous injectionthrough the caudal vein, and DNAs were extrdcted from the human hepatoma or the normaltissues of nude mice at various time intervals after inoculation. The results showed that H-1replication was found exclusively within the confines of the transplanted human hepatoma butnot in any of the normal tissues of the nude mice.

关 键 词：肝癌 细小病毒H-1 DNA 聚合酶链反应 复制 

分 类 号：R735.702[医药卫生—肿瘤] R373.9[医药卫生—临床医学]