茶多酚对鼠心肌缺血再灌产生氧自由基的清除  

SCAVENGING EFFECTS OF GREEN TEA POLYPHENOLS ON THE OXYGEN FREE RADICALS GENERATED FROM ISOLATED ISCHEMIC REPERFUSED RAT MYOCARDIUM

作　　者：邹曦露[1] 万谦[1] 李美芬[1] 赵保路[1] 忻文娟[1] 

机构地区：[1]中国科学院生物物理研究所

出　　处：《波谱学杂志》1995年第3期237-244,共8页Chinese Journal of Magnetic Resonance

摘　　要：用电子顺磁共振（ＥｌｅｃｔｒｏｎＳｐｉｎＲｅｓｏｎａｎｃｅ，缩写ＥＳＲ）在低温１１０Ｋ条件下，以离体大鼠心脏缺血再灌为模型，直接测量了缺血再灌损伤时产生的活性氧自由基信号．实验发现，缺血再灌损伤的心肌中氧自由基的含量比正常灌流心肌增加了１８８％，茶多酚可以使其降低７１％。这说明茶多酚对氧自由基引发的缺血再灌损伤有保护作用。Electron spin resonanoe（ESR）has been applied to direct measurement of free radical generation in ischemic perfused rat myocardium at 110K. The results show that the isolated perfused rat heart subjected to 30－minute ischemic followed by 15－second reperfusion induced a significant increase of oxygen free radicals production. It was found that the active oxygen free radicals were about 188 ％ more in ischemic reperfused than those in normal perfused.After pretreatment of the heart with GTP，the oxygen free radicals can be reduced by 71％。 These results might indicate that the natural antioxidants GTP can protect the heart from the injuries by ischemic reperfusion.

关 键 词：氧自由基 缺血再灌 茶多酚 顺磁共振 心肌缺血 

分 类 号：R972.9[医药卫生—药品] O482.533[医药卫生—药学]