原发性骨肿瘤细胞核DNA含量的流式细胞光度分析  

FLOW CYTOMETRIC ANALYSIS OF DNA IN BONE TUMORS USING NUCLEAR SUSPENSION

作　　者：尹格平[1] 刘振华[1] 何德华[1] 左连富[2] 

机构地区：[1]第二军医大学病理解剖学教研室 [2]河北省肿瘤研究所

出　　处：《第二军医大学学报》1989年第5期417-423,共7页Academic Journal of Second Military Medical University

摘　　要：用流式细胞光度分析术对33例原发性骨肿瘤细胞核DNA含量进行了研究。采用简便、快速、高效的方法处理经10％福尔马林固定、石蜡包埋的肿瘤组织,使成为单细胞核悬液。结果表明:10例良性骨肿瘤(包括5例Ⅰ级骨巨细胞瘤)和5例组织学分级有疑问的肿瘤(又称交界性肿瘤)。细胞核为正常倍体DNA含量;7例交界性肿瘤和11例恶性骨肿瘤为异倍体DNA含量,细胞周期分布也表明,异倍体肿瘤的S期及M+G2期细胞数比2倍体和近2倍体肿瘤的多。DNA含量的类型可分4种:(1)2倍体和近2倍体;(2)3倍体左右的异倍体;(3)4倍体DNA含量;(4)双DNA干系。本实验还证明用10％福尔马林固定、石蜡包埋的组织进行流式细胞分析方便可行,特别适用于少见病例。The types of DNA content can be divided into four groups: (1) diploids and near-diploids; (2) triploids; (3) tetraploidy and hyperploidy aneuploids; (4) biclonal DNA content.Recent studies show that measuring DNA content by flow cytometry (FCM) can be applied to most primary bone tumors in pointing out clinically relevant informations.In this study, cellular DNA content of 33 primary bone tumors was analysed by FCM.Isolated nuclear suspensions were prepared by a simple, rapid and effective method using 10% formalin-fixed and paraffin-embedded bone tumor specimens.The results showed that 10 benign (including 5 Grade I giant cell tumors of bone) and 5 histologically questionable tumors had normal DNA content (diploids or near-diploids) and that the other 7 questionable and all of the 11 malignant tumors had abnormal DNA content (aneuploids).The cell cycle distribution analysis showed that the aneuploidy tumors had higher proportion of S-phase and G2+M-phase cells than the normal ploidy tumors, indicating there were differences in proliferative activity.The method also showed that benign and low-malignant primary bone tumors were diploids or near-diploids, and high-malignant ones were aneuploids.Compared with typically pathological grading, the flow DNA analysis of bone tumors can more objectively point out their biological behavior and prognosis.

关 键 词：骨肿瘤 流式细胞分析 DNA 病理 

分 类 号：R738.102[医药卫生—肿瘤]