抗层粘蛋白（Laminin）杂交瘤细胞株的建立及临床应用  

Establishment and Application of Hybridomas Producing Anti-Laminin Monoclonal Antibody

作　　者：谢贤春[1] 吉中和[1] 皮国华[1] 张素香[1] 谷淑燕[1] 王慧娟[1] 邓瑶[1] 

机构地区：[1]北京佑安医院肝炎研究所,中国预防医学科学院病毒学研究所

出　　处：《单克隆抗体通讯》1995年第3期44-47,共4页

摘　　要：本文用纯化的层粘蛋白作为免疫原免疫ＢＡＬＢ／ｃ小鼠，取脾细胞与Ｓｐ２／０小鼠骨髓瘤细胞融合，获得５株能稳定分泌高效价抗层粘蛋白单克隆抗体杂交瘤细胞株。并建立了检测血清中层粘蛋白的简便、快速和稳定的酶联免疫吸附试验．用其建立的检测技术，检查了急性肝炎、慢迁肝、慢活肝、肝硬化病人和正常人血清中的层粘蛋白含量。表明除慢迁肝外，各型肝炎及肝硬化病人血清层粘蛋白的浓度明显高于正常人，病人血清中层粘蛋白升高与肝脏纤维化程度呈正相关．本研究为诊断肝纤维化、肝硬化及门脉高压症等疾病提供了一种血清学指标。Monoclonal antibodies (McAbs) against laminin were produced by fusing Sp2/0 myeloma cells with spleen cells from BALB/c mice immunized with laminin.Five hybridoma cell lines secreting specific antibodies against laminin were obtained.These McAbs were proved to be monospecific by ELISA.This McAb mixture could be preserved with liquid nitrogen for at least 1 year without any loss in immune reactivity. Comparison of the serum laminin between normal individuals and patients with various hepatopathy showed a positive correlation between elevation of serum laminin and extent of the liver cirrhosis.

关 键 词：肝硬变 层粘蛋白 单克隆抗体 杂交瘤 血清诊断 

分 类 号：R575.204[医药卫生—消化系统] R392－33[医药卫生—内科学]