电针刺激大鼠足三里穴对视上核、室旁核催产素神经元的影响  

Effect of electroacupuncture of "Zusanli" acupoint on the oxytocin -immunoreactive cells of the supraoptic nucleus and paraventricular nucleus of hypothalamus in rats

作　　者：何润昌 范溥生 王旭明[1] 何北平[1] 张忠义[1] 周世臣[1] 宋朝佑[1] 林葆城[1] 

机构地区：[1]第二军医大学基础医学部解剖学教研室,第二军医大学基础医学部神经生物学教研室

出　　处：《第二军医大学学报》1995年第1期37-40,共4页Academic Journal of Second Military Medical University

基　　金：国家自然科学基金

摘　　要：用ＷＱ－９Ｅ钾离子透入仪筛选出对痛刺激敏感的ＳＤ大鼠８只，随机分成对照组及针刺组，每组４只。针刺鼠用ＷＱ－９Ｅ电针仪刺激两侧＂足三里＂穴，持续３０ｍｉｎ。所有动物同时用１％戊巴比妥钠腹腔内麻醉后开胸，用Ｂｏｕｉｎ液经心灌注固定，开颅取脑，作连续冰冻切片（片厚４０μｍ），隔片取一。所有切片均按Ｓｔｅｒｎｂｅｒｇｅｒ（ＰＡＰ）法进行免疫细胞化学反应，ＤＡＢ呈色，封片后直接连ＩＢＭ２８６－ＣＡＰ５３０图像分析系统对视上核，室旁核催产素免疫反应阳性物质作定量测定。结果显示：针刺鼠下丘脑视上核，室旁核催产素免疫反应阳性物质的总面积比对照鼠分别增加了４４．１％、４７．３％（Ｐ＜０．０１）。提示，催产素免疫反应阳性物质的增加与电针刺激足三里穴有关，催产素可能参与电针镇痛的中枢调节过程。Eight adult male Sprague-Dawley rats sensitive to pain induced by potassium ionophoresis were randomly divided into experimental group and control group. There were four rats in each group. The experimental rats were electroacupunctured 'Zusanli' for 30min. The control rats remained unstimulated. All the experimental and control rats were then aneshetized with sodium pentobarbital, perfused transcardially with Bouis's fixative solution. Then the brains were removed and cut coronally with a freezing rotary microtome (40 μm thick). All the sections of two groups were incubated in anti oxytocin serum and processed for immunostaining by applying the peroxidase-antiperoxidase (PAP) method of Sternberger's.The total areas of oxytocin-immunoreactive (OT-ir) substance of the supraoptic nucleus (SON) and paraventricular nucleus (PVN) on the same side of all sections were measured by a microcomputer image analysis system (IBM286-CAP530). The result showed that the total areas of OT-ir substance of SON and PVN in the experimental rats were significantly increased by 44.1% and 47.3% as compared with the control group respectively(P<0.01).Our data suggest that OT is possibly involved in the central modulation of acupuncture analgesia.

关 键 词：电针 视上核 室旁核 催产素 免疫细胞化学 

分 类 号：R322.81[医药卫生—人体解剖和组织胚胎学]