抗人肝癌单克隆抗体在裸鼠移植瘤中的免疫定位  

Radioimmunological image of anti-human primary hepatic cancer monoclonal antibody in tumor-bearing nude mice

作　　者：施乐华[1] 吴孟超[1] 陈汉[1] 陶文照[1] 强美玉[1] 谢弘[1] 王根风[1] 王放[1] 

机构地区：[1]第二军医大学长海医院肝胆外科,第二军医大学基础医学部病理解剖学教研室,第二军医大学海军医学系放射医学教研室,中科院上海细胞生物学研究所

出　　处：《第二军医大学学报》1995年第2期127-129,共3页Academic Journal of Second Military Medical University

摘　　要：以１３１Ⅰ标记抗人肝癌单克隆抗体（Ｈｅｐａｍａ－Ⅰ，以下简称［１３１Ⅰ］－Ｈｅｐ）及正常小鼠ＩｇＧ（以下简称［１３１Ⅰ］－ｍＩｇＧ）进行裸鼠人原发性肝癌移植瘤的肿瘤定位显像研究。每鼠从尾静脉注射１．１１×１０７Ｂｑ，注入后２４，４８，９６，１６８ｈ行全身γ照相，观察肿瘤摄取标记抗体的能力，并计算生物半衰期，同时定期测定肿瘤与各器官组织的放射性比值（Ｔ／ＮＴ）。结果：［１３１Ⅰ］－Ｈｅｐ能选择性地积聚于肿瘤组织，其摄取率高于［［１３１Ⅰ］－ｍＩｇＧ（Ｐ＜０．０５），Ｔ／ＮＴ值在注入后９６ｈ最高，［１３１Ⅰ］－Ｈｅｐ的体内存留时间明显长于［１３１Ⅰ］－ｍＩｇＧ。提示Ｈｅｐａｍａ－Ⅰ在裸鼠体内对人肝癌细胞具有较高的亲和力，是一种有用的导向载体。Tumor xenografts of human primary hepatic cancer (PHC) were grown in nude mice subeutaneously. The studies were performed in nude mice with tumor of base near 0.8~ 1.0 cm3. For the study of RII,the mice were injected intravenously with 1. 11×107Bq/mouse of [131I]-Hep. All tumors were clearly visualied at the 4th day after injection and remained the same at the 7th day. No tumor imasing was found in [131I]-mIgG.The radioactive ratio of tumor to liver increased from 1.3 to 3.03 in the [131I]-Hep group, but decreased from 13. 6 to 6.88 in the [131I]-mlgG group.

关 键 词：单克隆抗体 肝肿瘤 放射性核素 成像 

分 类 号：R735.703[医药卫生—肿瘤]