N－乙基－N－亚硝基脲诱发大鼠胶质瘤的实验研究  

A study to induce brain glioma with N-ethyl-N-nitrosourea in rats

作　　者：卞修武[1] 史景泉[1] 辛榕[1] 陶海鹏[1] 杨光华[1] 

机构地区：[1]第三军医大学基础部病理学教研室,华西医科大学药学院有机化学教研室,华西医科大学病理学教研室

出　　处：《第三军医大学学报》1995年第1期29-32,共4页Journal of Third Military Medical University

基　　金：国家自然科学基金

摘　　要：采用人工合成的Ｎ－乙基－Ｎ－亚硝基脲（ＥＮＵ，６０ｍｇ／ｋｇ体重），经胎盘和皮下给药分别在妊娠晚期孕鼠子代和３天龄Ｗｉｓｔａｒ大鼠经１２个月诱发出神经系统胶质瘤．成瘤率分别为７３．２％和６８．３％（胶质瘤为６５．９％和６３．４％）；胶质瘤以少突－星形细胞瘤和少突胶质细胞瘤为主，呈小灶性、多灶性、混合性，并伴有灶性和／或弥漫性胶质增生存在．结果表明，这两种胶质瘤模型为深入研究胶质瘤的发生、生长及分化提供了良好的途径．本文讨论了灶性胶质母细胞增生在胶质瘤发生学上的意义．In order to induce brain glioma, transplacental and subcutaneous injections of N-ethyl-N-nitrosourea(ENU,60 mg/kg body weight)were given to the Wistar rats in late gestational stage and of 3 days of age.The rats were followed up until the end of the l2th month after drug administration.The rate of total tumor formation was 73.2% and 68.3% and that of brain glioma was 65.9% and 63.4% respectively, Histologically,the main types of glioma were mixed oligodendro-astrocytoma and oligodendroglioma.The tumors exhibited the microfocal,multifocal and mixed features with focal and/or diffuse proliferation of glial cells.These findings indicate that the 2 rat models of glioma induced with synthetic N-ethyl-N-nitrosourea were successful and provide a reliable approach for the study of the pathogenesis of cerebral glioma.In addition,the significance of the proliferation of focal glioblasts in the genesis of cerebral glioma was discussed.

关 键 词：胶质瘤 乙基亚硝基脲 大鼠 动物模型 病因 

分 类 号：R730.264[医药卫生—肿瘤]