环境致癌物阻断NIH3T3细胞间隙连接通讯与细胞内游离钙离子关系初探  

Prelminary study of the relationship of the blockade on gap junctional interceular communication by environmenta carcinogens with cytosollic ree calcium ion ocncentration in NIH 3T3 cells in vitro

作　　者：张晓丽[1] 高宁[1] 卓鉴波[1] 邱志群[1] 曹波[1] 陈东升[1] 

机构地区：[1]第三军医大学预防医学系环境卫生学教研室

出　　处：《第三军医大学学报》1995年第5期405-407,共3页Journal of Third Military Medical University

摘　　要：选用环境致癌物芥子气（ＭＧ）、苯并（ａ）芘（Ｂ（ａ）ｐ）、４－硝基喹啉－１－氧化物（４ＮＱＯ）、二甲基亚硝胺（ＤＥＮ）、环磷酰胺（ＣＰ）、雌二醇（Ｅｓｔ）、四氯化碳（ＣＣｌ４），作用于该细胞１２ｈ后，用划痕标记染料示踪技术（ＳＬＤＴ）检测其对该细胞间隙连接通讯（ＧＪＩＣ）功能的阻断作用，同时测定该细胞内［Ｃａ２＋］ｉ的变化，结果表明，环境致癌物阻断细胞ＧＪＩｃ与［Ｃａ２＋］ｉ变化密切相关。Seven environmental carcinogens including mustard gas,benzo(a)oyyrene,4-nitroquinoline-1-oxide,nitrosodimethylamine,cyclophosphamide,carbon tetrachloride and estradiol were sected for the study.12 hours after the administration of the carcinogens,to NIH3T3 cells in vitro,scrape-loading and dye transfer was used to observe the gap junctional intercellular communication and cytosoic free calcium ion concentration(Ca2+)in NIH3T3 cells was determined.It was found that all the 7 environmental carcinogens blocked the gapjunctional intercelluar communication which was closely related to the Ca2+ level of NIH3T3 cells.

关 键 词：细胞间隙 连接通讯 钙离子 环境致癌物 

分 类 号：R730.2[医药卫生—肿瘤]