雌激素对乳腺癌细胞的生长及p185蛋白和表皮生长因子受体表达的影响  

The influence of estradiol on growth of breast cancer ce11s and ex-pression of pl85 and epidermal growth factor receptor

作　　者：郝新保[1] 张盈华[1] 殷缨[1] 王莉莉[1] 丘福海[1] 

机构地区：[1]唐都医院中心实验室,唐都医院普通外科

出　　处：《第四军医大学学报》1995年第1期46-48,共3页Journal of the Fourth Military Medical University

摘　　要：为了研究乳腺癌的发展过程中雌二醇（Ｅ＿２）的作用机制及参与因素，作者使用流式细胞仪观察了人乳腺癌细胞株ＳＫ－ＢＲ－３在Ｅ＿２作用下的生长特征及ｐ１８５蛋白和表皮生长因子（ＥＧＦ）受体的表达．结果表明，乳腺癌细胞生长加快，ＤＮＡ合成增加，Ｓ期百分率升高（Ｅ＿２组２６．７±２．５，对照组２１．２±２．１，Ｐ＜０．０５）．在Ｅ＿２的作用下，乳腺癌细胞的ｐ１８５蛋白表达降低（Ｅ＿２３５±５．６，对照组６１±１３．１，Ｐ＜０．０５）而ＥＧＦ受体表达升高（Ｅ＿２３９±６．９，对照组２１±５．４，Ｐ＜０．０５），提示ＥＧＦ在乳腺癌的早期发展中可能发挥更主要的作用．In order to study the mechanism of estradiol and other events involved in development of breastcancer, we observed the growth as well as the expression of p185 and epidermal growth factor(EGF)recep-tor in human breast cancer cell line SK-BR-3 at the prenence of estradiol used flow cytometry. The resultsshowed that cells treated with estradiol were induced proliferation and DNA synthesis, the percentage of Sphase raised(E_2 group 26.7±2.5,control group 21.2±1, P<0.05).The reduction of p185 expression(E2 35±5.6,control 61±11, P<0.05) induced by estradiol was accompanied by a strong enhancement ofEGF receptor expression E_2 39±6.9,control 21±5.4, P<0.05).It is suggested that EGF may play aquite important role in early developing of breast cancer.

关 键 词：乳腺癌 雌二醇 NEU基因 表皮生长因子 受体 

分 类 号：R737.902[医药卫生—肿瘤]