外磁场引导定位秋水仙碱－磁微粒对裸鼠乳腺癌的抑制作用  

Inhibitory effect of colchicine albumin magneticrosphere with ex-ternal magnetic orientation on mamary cancer in nude mice

作　　者：张仲海[1] 吴少华[1] 夏天[1] 朱德生[1] 赵建斌[1] 王文学 

机构地区：[1]西京医院中医科

出　　处：《第四军医大学学报》1995年第2期114-116,共3页Journal of the Fourth Military Medical University

基　　金：国家自然科学基金

摘　　要：作者用白蛋白包埋法制成具有磁响应的秋水仙碱－白蛋白－磁微粒，并采用ＭＴＴ法检测了秋水仙碱－白蛋白－磁微粒及游离秋水仙碱对ＥＭＴ＿６细胞的生长抑制作用，对乳癌ＥＭＴ＿６细胞裸鼠移植瘤进行基底部注射和外加磁场定位治疗．结果表明：秋水仙碱－白蛋白－磁微粒及游离秋水仙碱在体内、体外实验均对肿瘤细胞有明显的杀伤作用，体外实验在１００μｇ／ｍｌ剂量时，灭活率分别为５６．１６％和５８．４６％．对乳癌ＥＭＴ＿６细胞裸鼠移植瘤的抑瘤率分别为３２．８％和２３．６％，提示秋水仙碱－磁微粒外加磁场引导定位对裸鼠乳腺癌有较好的抑制作用．In the present study albumin-embedded colchicine albumin magnetic microsphere(Fm-HSA-Co)was prepared. MTT was employed to determine Fm-HSA-Co and free colchicine’s inhibition of EMT_6 cellgrowth.Basilar injection and external magnetic orientation therapy were performed on mammary cancerEMT-6 cell xenografts in nude mice.The results showed:Fm-HSA-Co and free colchicine caused evident de-struction of tumor cells both in vitro and in vivo.In the in vitro experiment,the inhibition rates were respec-tively 56.16%and 58.46%(P<0. 01 vs control)at the dose of 100 μg/ml, while in vivo the tumor-inhibit-ing rates against mammary cancer EMT_6 cell xenografts in nude mice were 32.8%and 23.6%(P<0.05 vscontrol),suggesting that with the assistance of external magnetic orientation colchicine albumin magnetic mi-crosphere has stronger inhibition to breast tumor in nude mice.

关 键 词：乳腺癌 磁微粒 定位 秋水仙碱 治疗 抗癌药 

分 类 号：R737.905[医药卫生—肿瘤] R979.1[医药卫生—临床医学]