LAK细胞在单克隆抗体介导下的抗癌作用  

Lymphokine-activated killer cells mediated monoclonal antibodyagainst gastric cancer

作　　者：毕锋[1] 张学庸[1] 牟震先[1] 吴觉平[1] 樊代明[1] 胡家露[1] 

机构地区：[1]西京医院消化内科

出　　处：《第四军医大学学报》1995年第2期102-104,共3页Journal of the Fourth Military Medical University

摘　　要：目的：探讨增强ＬＡＫ细胞抗肿瘤作用的新方法．方法：用健康人外周血经密度梯度离心法分离出单个核细胞，再加入白细胞介素２（ＩＬ－２）后诱导的ＬＡＫ细胞为效应细胞，采用４－ｈ￣５１Ｃｒ释放法检测了其对胃癌细胞ＫＡＴＯⅢ的杀伤作用及加入抗胃癌单克隆抗体ＭＧｂ２后的影响．结果：发现利用单抗ＭＧｂ２和ＬＡＫ细胞产生的抗体依赖细胞介导的细胞毒性作用（ＡＤＣＣ）可以明显增强ＬＡＫ细胞的抗胃癌作用（约１００％，Ｐ＜０．０１）．此作用在单克隆抗体浓度为０．０１ｍｇ／Ｌ时出现，２ｍｇ／Ｌ时达到高峰．制备ＬＡＫ细胞时ＩＬ－２的浓度，不仅影响ＬＡＫ活性，也影响着相应的ＡＤＣＣ效应．结论：利用单抗ＭＧｂ２和ＬＡＫ细胞产生的ＡＤＣＣ效应可以明显增强ＬＡＫ细胞的抗胃癌作用．Objective:To probe for a new way of enhancing lymphokine-activated killer(LAK)cells against carcinoma.Methods : LAK cells prepared with mononuclear cells(separated from normal human peripheral blood using gradient density centrifugation)induced by interleukin-2 (IL-2)were taken as effector cells.The 4h ￣51Cr release assay was employed to determine the killing capacity of the effector cells to gastric cancer cells KATO Ⅲ prior to and during the mediation of monoclonal antibody MGb2(McAB MGb2)to gastric can- cer.Results : The LAK cells against gastric cancer were found to be markedly reinforced(about 100%,P<0. 01)by the antibody-dependent cellular cytotoxicity (ADCC)resulting from the concurrent actions of McAb MGb2 and LAK cells.The reinforcement first occurred at 0.01 mg/L of McAb and climaxed at 2 mg/L.LAK activityand the corresponding ADCC were related with the IL-2 concentration for preparation of LAK cells.Conclusion :The combined use of LAK cells with McAb MGb2 can significantly enhance the cytotoxici- ty of LAK cells against gastric cancer.

关 键 词：胃肿瘤 LAK细胞 单克隆抗体 

分 类 号：R730.51[医药卫生—肿瘤]