四种植物多糖对S_（180）细胞膜磷脂酰肌醇转换的影响  

Effect of plant polysaccharides on phosphatidylinositol turnover of the plasma membrane of Sarcoma S_180 cell

作　　者：黄添友[1] 佟丽[1] 吴波[1] 梁谋[1] 梁念慈 李吉来[1] 

机构地区：[1]第一军医大学中西医结合研究所,广东医学院

出　　处：《第一军医大学学报》1995年第3期211-212,共2页Journal of First Military Medical University

基　　金：1992年度全军青年科学基金

摘　　要：将茯苓多糖（ＰＰＳ）、刺五加多糖（ＡＳＰＳ）、银耳多糖（ＴＦ）、香菇多糖（Ｌｅｎｔｉｎａｎ）与Ｓ＿１８０细胞膜、（γ－￣３２Ｐ）ＡＴｐ－同温育，抽提并分离肌醇磷脂进行放射自显影和液闪计数。结果表明茯苓多糖，刺五加多糖对Ｓ＿１８０细胞膜网脂酰肌醇（ＰＩ）转换有显著抑制作用，刺五加多糖对其抑制作用与剂量呈正相关。银耳多糖、香菇多糖对ＰＩ转换无影响。Mice sarcoma S_180 cell membranes,[γ-32]ATP and plant polysaccharide(PPS,ASPS,TF orlentinan)were incubated together for 5 min at 30 ℃,Phosphatidylinositides were extracted and isolated,and radioactivity was measured with liquid scintillation counter.The results showed that PPS and ASPS,especially the latter,could inhibit the phosphatidyinostol turnover obviously,while TF and lentinan hadno such effect Combining with our previous findings that PPS and ASPS could inhibit the proliferation ofS_180 cell significantly while TF and lentinan had no such effect,we conisider that the antitumor mechanismof plant polysaccharides(PPS and ASPS)may be related to the inhibition of phosphatidyinositol turnover.

关 键 词：植物多糖 肉瘤 磷脂酰肌醇 伏苓 银耳 香菇 

分 类 号：R285.5[医药卫生—中药学] R730.52[医药卫生—中医学]