丁酸引起人肺巨细胞癌胞核超微结构变化的定量分析——数字图象处理  

Quantitative Analysis of Butyric Acid-Induced Nuclear Ultrastructual Alterations of Human Lung Giant Cell Carcinoma Cells by Digital Image Processing

作　　者：仇玉福[1] 余立清[1] 朱庆华[2] 严慕陵[2] 

机构地区：[1]第三军医大学病理学教研室 [2]重庆大学无线电系

出　　处：《第三军医大学学报》1989年第6期443-4,共1页Journal of Third Military Medical University

摘　　要：本文旨在应用数字图象处理技术研究丁酸对人肺巨细胞癌PLA-801D细胞株胞核超微结构的影响。癌细胞在含2 mmol丁酸的条件下培养4天后,同对照组相比,胞核的周长无明显变化,但胞核面积比对照组者约大1.4倍,说明丁酸处理后癌细胞核外形趋向规则。实验组胞核的光密度比对照组者明显降低(后者比前者大2.7倍),表明丁酸作用后胞核中的染色质颗粒减少。结果提示丁酸可能具有促使恶性细胞胞核的某些恶性表型逆转化的生物学效应。The effects of butyric acid (BA) on nuclear ultrastructure of human lung giant cell carcinoma (Strain PLA-801 D) were studied with the digital image processing.It was found that the length of the nuclear circumference of the tumor cells incubated with 2mmol BA for 4 days was approximately equal to that of the control, whereas the nuclear area increased 1.4 times. It suggested that the nuclear profile tended to decome more regular. In addition, the nuclear optical density in the experimental group decreaed significantly compring with that of the control. This finding indicated that the chromatin granules within the nuclei were decreased by virtue of BA.BA may have a biological effect of reverse-transformation on the malignant cells.

关 键 词：丁酸 肺巨细胞癌 生物学效应 

分 类 号：R730.213.1[医药卫生—肿瘤]