蛋白质在NDG系列色谱填料中吸附平衡的研究  

A Study on the Adsorption Equilibrium of Protein on the NDG Series Chromatographic Packing

作　　者：王志祥[1] 钱杰生[1] 何志敏[1] 余国琮[1] 

机构地区：[1]东南大学化学化工系,天津大学化学工程研究所

出　　处：《东南大学学报（自然科学版）》1995年第3期134-138,共5页Journal of Southeast University：Natural Science Edition

基　　金：国家教委留学回国人员工作资助费支持项目。

摘　　要：蛋白质在ＮＤＧ系列色谱填料中吸附平衡的研究王志祥，钱杰生，何志敏，余国琮（东南大学化学化工系，南京２１００１８）（天津大学化学工程研究所，天津３０００７２）关于蛋白质在ＮＤＧ系列色谱填料中的吸附平衡，目前尚未见报道。蛋白质分子多为生物大分子，结构复杂...In this paper, the adsorption equilibrium relationshipe of bovine serum albumin and bovineblood hemoglobin on the NDG series chromatographic packing are studied in detail.The results showthat the adsorption equilibrium can be described by both Langmuir equation and Freundlich equationand the packing slze haslittle effect on the equilibrium adsorptive capacity,but the size of the hole di-ameter in the packing can notably influence the capacity of equilibrium adsorption. With the increaseof the size of the hole diameter in the packing,the equilibrium adsorptive capacity gradually increasesuntil reaching the maximum,then gradually drops.

关 键 词：蛋白质 色谱填料 吸附平衡 色谱分离 

分 类 号：O629.73[理学—有机化学] TQ028.3[理学—化学]