四膜虫细胞的核骨架及类中间纤维  

NUCLEAR MALRIX-INTERMEDIATE FILAMENT SYSTEM IN TELRAHYMENA THERMOPHILA

作　　者：蔡树涛[1] 焦仁杰[1] 史晓露 翟中和 

机构地区：[1]北京大学生物学系,北京100871

出　　处：《动物学报》1995年第2期212-217,共6页ACTA ZOOLOGICA SINICA

基　　金：国家自然科学基金

摘　　要：采用非树脂包埋去包埋剂超薄切片结合选择性生化抽提方法显示，原生动物四膜虫细胞大核具有发达的核骨架纤维网络，核周是一层完整的核纤层结构，在四膜虫细胞小核中，亦存在核骨架和核纤层。四膜虫细胞皮层中存在水不溶性纤维网架，其中含有类中间纤维蛋白组分，４９ＫＤ蛋白。Using selective extraction together with embedment-free electron microscopy techniques the nuclear matrix in macronuclei of Tetrahymena thermophila was displayed, where the inner nuclear matrix was bounded by a typical layer of nuclear lamina. There also exists nuclear matrix and lamina in micronuclei of T. thermophila cell. Immunoblot analyses showed that the 49KD protein in T. thermophila cross-reacted with mammalian anti-vimentin polyclonal antibodies and anti-lamins monoclonal antibodies. SDS-PAGE and protein isolation results showed that 49KD in T. thermophila cells is essential component of the nuclear matrix- intermediate filament system. Our results indicated that a nuclear matrix-intermediate filamentlike system similiar to that in mammalian nuclei appears in ciliated protozoan, T. thermophila. The authors propose that the nuclear matrix-intermediate filament system is not restricted to higher eukaryotic cells, and it may be originaled from the early stages of eukaryote evolution.

关 键 词：四膜虫 核骨架 中间纤维 核纤层 细胞 

分 类 号：Q959.117.3[生物学—动物学]