K_（562）细胞及K_（562）／ADM耐药细胞内cAMP及cGMP含量的测定  

Determination of the Levels of cAMP and cGMP in K(562) and K(562)/ADM Cell

作　　者：苏颖[1] 沈世人[1] 邹长棪[1] 高频[1] 

机构地区：[1]福建省肿瘤医院放射生物研究室

出　　处：《福建医学院学报》1995年第1期28-31,共4页

基　　金：福建省科委自然科学基金

摘　　要：测定正常人淋巴细胞内环磷腺苷（ｃＡＭＰ）、环磷鸟苷（ｃＧＭＰ）含量和人红白血病细胞株（Ｋ５６２）及其耐阿霉素细胞株（Ｋ５６２／ＡＤＭ）的增殖状况和细胞内ｃＡＭＰ、ｃＧＭＰ含量。结果Ｋ５６２与Ｋ５６２／ＡＤＭ细胞内ｃＡＭＰ含量均低于正常人淋巴细胞内ｃＡＭＰ，但无显著差异，Ｋ５６２与Ｋ５６２／ＡＤＭ之间细胞内ｃＡＭＰ的含量有显著差异，ｃＧＭＰ含量均未见差异。Ｋ５６２／ＡＤＭ细胞的增殖状况与ｃＡＭＰ含量呈负相关（ｒ＝－０．５８２３，Ｐ＜０．０５），加入逆转耐药的药物潘生丁、维拉帕米后，其增殖速度下降且差异显著（Ｐ＜０．０５），ｃＡＭＰ含量呈上升趋势；Ｋ５６２细胞也有此改变，但两种药物对两种细胞的影响程度不同。The levels of cAMP and cGMP in normal lymphocyte,K562 and K562/ADM cells were measured,and the rates of growth of K562 and K562/ADM were determined also.The results showed that,the levels of cGMP in three kinds of cell were not different,while the levels of cAMP in K562 and K562/ADM were lower then that in normal lymphocyte,but the difference was not significant.The difference of the level of cAMP in K562 and in K562/ADM was significant.For K562/ADM,negative correlation existed between the rate of growth and the level of cAMP,The growth rate decreased noticeably and cAMP level tended to increase after dipyridamole or verapamil were added.Same changs took place for K562 cell.However,the effects of two drugs on K562 and K562/ADM were different.

关 键 词：红白血病细胞株 K562 K562/ADM cAMP CGMP 

分 类 号：R73－3[医药卫生—肿瘤]