植物抗体基因工程：Ⅲ．马铃薯Y病毒小鼠中和抗体链基因的序列分析及其植物表达载体的构建  

Antibody Genetic Engineering in Plant:Ⅲ. Sequencing of a Kappa Light Chain Gene Against Potato Virus Y and Its Construction of Plant Expression Vector

作　　者：刘德虎[1] 陈三凤[1] 王海扬[1] 李刚强[1] 

机构地区：[1]中国农业科学院生物技术研究中心分子生物学室,北京农业大学生物学院微生物学系

出　　处：《高技术通讯》1995年第4期46-46,共1页Chinese High Technology Letters

基　　金：国家自然科学基金;863青年科学基金

摘　　要：序列分析表明，马铃薯Ｙ病毒小鼠中和抗体轻链基因（Ｋ６）全长为９５６个核苷酸碱基（不包括ｐｏｌｙ（Ａ）尾巴），其中包括５＇端非编码区３１个核苷酸碱基，编码轻链信号肽的５７个核苷酸碱基，编码成熟蛋白的６５７个核苷酸基和３＇端非编码区的２１１个核苷酸碱基。与已知的其它Ｋ轻链基因（ＭＲＫ１６）相比，核苷酸的序列同源性为９８．７％，由核苷酸序列所推导出的氨基酸序列同源性为９７．０％，其变异主要发生在三个抗原识别位点内（ＣＯＲ）。将完整的抗体轻链基因正向插入植物表达载体ｐＢ１２１中的原ＧＵＳ基因位置上，置于花椰菜花叶病毒３５Ｇ启动子控制之下，获得了能够在植物细胞内表达马铃薯Ｙ病毒中和抗体轻链基因的植物表达载体ｐＹＬ。Sequence analysis showed that the immunoglobulin messenger RNA corresponding to K6 consists of956 nucleotides in length excluding the poly(A)region,among which 31 bases cede for the 5' non-codingregion,57 for the leader sequence of the protein,657 for the mature protein and 211 other kappa lightchains show that they share a 100% identity in their constant regions(CL)and 93.7% identity in theirvariable regions(VL),The kappa light chain enceded by K6 was considered to be specific to PVY sinceonly one type of light chain was expressed in the hybridoma. A binary vectot, pYL,containing the lightchain gene has been constr ucted for its expression in higher plants by in treduction of the gene codingsesquence into pB121 instead of GUS gene.

关 键 词：马铃薯Y病毒 单克隆抗体 基因工程 植物抗体 

分 类 号：S188[农业科学—农业基础科学]