耐碱性芽孢杆菌碱性淀粉酶的研究——Ⅱ.菌株的诱变与产酶条件  

Studies on Alkaline Amylase from Alkaliphilic Bacillus megaterium——Ⅰ . Selection of High Producing Strain and Conditions for Enzyme Production

作　　者：贾士儒[1] 赵树欣[1] 黎京华 陈晓莉 

机构地区：[1]天津轻工业学院

出　　处：《工业微生物》1995年第1期13-16,共4页Industrial Microbiology

基　　金：天津市21世纪青年基金资助课题

摘　　要：耐碱性巨大芽孢杆菌(Bacillus megaterium)9-A2经紫外线、甲基磺酸乙酯和硫酸二乙酯等多次诱变处理,获得一株产碱性α-淀粉酶能力较高的变异株L-49。L-49菌株比出发株的产酶能力提高近2.5倍,当以酵母膏为氮源,可溶性淀粉为碳源,初始pH9～10,种龄18h,接种量5％(V/V),30℃培养48h,酶活力可达730u/ml。A mutant strain L-49 with high yield of alkaline α-amylase was obtained from alkaliphilic Bacillus megaterium 9-A2 by treatments of EMS and DES. The enzyme activity was improved from 280u/ml to 730u/ml (assayed at 40°C pH9). The optimum conditions for enzyme production were as follows, yeast extract as nitrogen source, starch as carbon source, initial pH 9-10, an inoculum size of 5%, and incubated at 30°C for 48h.

关 键 词：碱性α-淀粉酶 耐碱性芽孢杆菌 

分 类 号：Q939.110.6[生物学—微生物学]