二倍体乳腺癌细胞的流式细胞计量术分析  

Flow Cytometric Study on Diploidy Breast Cancer Cells

作　　者：周振英[1] 张军妮[1] 赵祥生[1] 宋心龙 林琳[1] 高萍[1] 

机构地区：[1]江苏省肿瘤防治研究所

出　　处：《河南肿瘤学杂志》1995年第2期81-83,共3页Henan Journal of Oncology

摘　　要：作者用流式细胞计量术检测了３９例二倍体乳腺癌细胞的增殖活性和非克隆性异倍体细胞的分布。结果表明，二倍体乳腺癌细胞的ＳＰＦ、ＰＩ、＞２Ｃ％、＞４Ｃ％、ＤＮＡ量值分布、Ｇ（０／１）峰ＣＶ值和Ｇ（０／１）峰倾斜度等指标均明显高于正常对照组（Ｐ＜０．０１或Ｐ＜０．０５）。这些指标与患者淋巴结转移和临床分期的关系密切。淋巴结转移阳性病例的ＳＰＦ、＞２Ｃ％、＞４Ｃ％、Ｇ（０／１）峰ＣＶ值和Ｇ（０／１）峰倾斜度均显著高于淋巴结转移阴性者（Ｐ＜０．０５）。随临床分期增加，其＞４Ｃ％呈逐渐增高的变化（ｒ＝０．９９３，ｒ＞ｒ（０．０５））。这表明，二倍体乳腺癌细胞生物学特性与正常对照细胞有明显不同。它不仅有较高的增殖活性，而且还含有一些非克隆性异倍体细胞。Proliferous activity and non-clone aneuploidy of tumor cells were measured by flowcytometric method on 39 patients with diploidy breast cancer.The results showed that SPF(S-Phase fraction),PI (proliferous mdes),>2C% (high diploidy cells percentage),>4C%(high tetraploidy cells percentage),distributing limit of cellular DNA content,CV anddeviated degree of diploidy cells G0/1 peak in diploidy tumor were higher than those in control(P<0.01 or P<0. 05).These targets of the patienst were closely related to LNM andclinical stages of disease. SPF,>2C%,>4C%,CV and deviated degree of G0/1peak in LNM(+)cases were higher than those in LNM(-)cases(P<0.05).The 4C% increased as theclinical stages of disease in diploidy breast cancer patients went up (r=0.993,r>r0.05).Thisshowed that the biological characteristics of diploidy tumor cells were quite differrent withthose of normal diploidy cells.They had not only higher proliferous activity, but also noncline aneuploidy cells.

关 键 词：流式细胞计量术 乳腺癌 脱氧核糖核酸 细胞干系 

分 类 号：R737.904[医药卫生—肿瘤]