霉烂水果优势真菌提取物致突变性研究  

Study on the mutagenicity of the extracts from preponderant fungi in mildewed fruits

作　　者：杨胜利[1] 宋爱云[1] 韩绍印[1] 丁兰萍[1] 马刚军[1] 

机构地区：[1]河南医科大学食管癌研究室

出　　处：《河南医科大学学报》1995年第1期10-12,共3页Journal of Henan Medical University

基　　金：河南省卫生厅资助

摘　　要：用细菌回复突变试验和小鼠骨髓嗜多染红细胞（ＰＣＥ）微核试验，研究了经分离获得的引发梨霉烂的优势真菌互隔交链孢霉（Ａ．ａｌｔｅｒｎａｔａ）及引发桔子霉烂的优势真菌指状青霉（Ｐ．ｄｉｇｉｔａｔｕｍ）提取物的致突变性。结果表明：所测试的３株互隔交链孢霉（Ａ_１、Ａ_２、Ａ_３）提取物及４株指状青霉（Ｐ_１、Ｐ_２、Ｐ_３、Ｐ_４）提取物均可不同程度地诱发Ｅ．ｃｏｌｉＮＤ－１６０菌株回复突变。且其中Ａ_１、Ａ_２和Ｐ_２、Ｐ_４株提取物还可诱发小鼠骨髓ＰＣＥ微核率明显升高（Ｐ＜０．０１）。结果提示分别引发梨及桔子霉烂的互隔交链孢霉及指状青霉其代谢产物均具致突变性。With reverse mutation assay and micronucleus test. we studied the mutagenicity of the extracts of Alternaria alternata and Penicillium digitatum, the preponderant strains isolated from mildewed oranges and pears respectively.The results showed that the extracts of Alternaria alternata strains(A1,A2,A3) and Penicillium digitatum(P1,P2,P3,P4) induced the reverse mutation of E. Coli ND-160 strain,and the extracts of A1,A2,P2and P4 strains also significantly induced the increase of micronucleus rate of mouse bone marrow cells(P<0.01). This indicated that the metabolites of Alternaria alternata and Penicillium digitatum from mildewed oranges and pears possessed mutagenicity.

关 键 词：水果 真菌 致突变性 

分 类 号：R155.54[医药卫生—营养与食品卫生学] R994.1[医药卫生—公共卫生与预防医学]