食管癌高发区人群食管癌前病变癌基因EGFR、C－Jun和Ras蛋白表达的研究  

Expression of EGFR, C-Jun and Ras protein in human esophageal precancerous lesions from subjects in high-risk area of esophageal cancer in Henan

作　　者：王立东[1] 周琦[1] 王耀红[1] 邢莹[1] 李启驹[1] 裘宋良[1] 

机构地区：[1]河南医科大学医学实验中心癌症研究室,河南省肿瘤病理学重点实验室,河南医科大学第一附属医院病案室,新乡医学院生理学教研室,新乡市中心医院肿瘤外科

出　　处：《河南医科大学学报》1995年第2期143-145,共3页Journal of Henan Medical University

基　　金：国家自然科学基金;美国NCI联合资助

摘　　要：为进一步了解食管癌前病变发生的分子基础，采用免疫组织化学技术测定人食管正常上皮和癌前病变组织中表皮生长因子受体ＥＧＦＲ，Ｃ－Ｊｕｎ和Ｒａｓ三种癌基因蛋白的表达状况。结果：在５４例食管粘膜活检组织中，共发现１２例正常上皮，３４例基底细胞增生和８例间变。在２９例ＥＧＦＲ测定中，正常组织未见免疫阳性反应，而基底细胞增生和间变分别出现３９％和８０％的免疫阳性反应率。在所有活检正常和癌前病变组织中未观察到Ｃ－Ｊｕｎ和Ｒａｓ蛋白表达。提示ＥＧＦＲ在食管癌前病变发生和发展中可能起一定的作用。The objective of this study is to investigate the molecular changes of human esophageal precancerous lesions by measuring the expression of EGFR, C-Jun and Ras proteins in the normal tissues and the tissues with different severities of lesions with immunohistochemistry method. Among the 54 subjects examined, there were 12 subjects with normal epithelium, 34 with basal cell hyperplasia and 8 with dysplasia. Immunoreactivity of EGFR was higher in dysplasia 98 (%) than in basal cell hyperplasia (39%). However, the normal epithelium did not show positive immunostaining. In the normal tissues and the tissues with different severity of lesion from esophageal biopsies, the expression of C-Jun and Ras protein was not detected. The findings indicate that EGFR may play a role in esophageal carcinogenesis. C-Jun and Ras protein niay not be an early event in esophageal carcinogenesis.

关 键 词：食管 癌前病变 癌基因 食管肿瘤 基因表达 

分 类 号：R735.102[医药卫生—肿瘤] R730.21[医药卫生—临床医学]