蝎毒对人肿瘤细胞株和动物移植性肿瘤的作用  

The effect of Scorpion venom on human tumor cell line and transplantable animal tumor

作　　者：郑智敏[1] 王成裕[1] 董伟华[1] 田中岭[2] 韩雪飞[2] 孔天翰[2] 

机构地区：[1]河南医科大学病理生理学教研室,郑州450052 [2]河南医科大学人体解剖学教研室,郑州450052

出　　处：《河南医科大学学报》1995年第4期351-354,共4页Journal of Henan Medical University

基　　金：河南省中医管理局重点资助课题

摘　　要：用人食管癌Ｅｃａ１０９、人宫颈癌ＨｅＬａ细胞株和艾氏腹水癌（ＥＡＣ）小鼠检测了马氏钳蝎 蝎毒（ＳＶＣ）体外、体内的抗肿瘤作用。结果显示，ＳＶＣ的浓度为０． ０１７ ｍｇ／Ｌ，处理Ｅｃａ １０９细胞２４、 ４８、７２ｈ后，细胞生长抑制率分别为 ３５． ６％、３９． ５％、３６， ９％；ＭＴＴ显色法表明，ＳＶＣ浓度为０． ０１７、 ０．０３４、０．０８５ ｍｇ／Ｌ时，对Ｅｃａ１０９细胞的毒性作用分别达 ６３％、５６％、５９％。 ＳＶＣ对体外培养的 ＨｅＬａ细胞作用不明显。 ＳＶＣ腹腔注射于ＥＡＣ小鼠，其剂量为每ｋｇ体重 ０． １、０． ３ ｍｇ，连续给药 １０ ｄ，ＥＡＣ小鼠的生命延长率分别为 ５２．０％、５４．４％，差异显著（Ｐ＜０． ０５）；在停药第 １０ ｄ时，ＥＡＣ小 鼠的体重抑制率亦有明显差异。结果证明：ＳＶＣ对Ｅｃａ １０９细胞和ＥＡＣ小鼠有抑制作用，对ＨｅＬａ 细胞作用不明显。The human cancer cell lines,HeLas and Eca 109 cells,were incubated with Scorpion venom crude (SVC). The mice with Ehrlich ascites carcinoma (EAC) were injected with SVC. The antineoplastic effects of SVC were studied in vitro and in vivo. The results showed that while Ecal09 cells were incubated with SVC for 24, 48,and 72h,the rates of inhibition were 35. 6%, 39. 5%, 36. 9%, respectively. The concentration of SVC used was 0. 017 mg/L. The results of MTT method indicated that when the concentration of SVC were 0. 017 mg/L, 0. 034 mg/L,and 0. 085 mg/L, the cytotoxicity were 63%, 56% and 59%, respectively. But SVC showed no apparent effect on HeLas. The EAC mice were injected with SVC 0.1 mg/kg and 0. 3 mg/kg for 10 d,and the rates of prolongated survival time of EAC mice were 52. 0% and 54.4% respectively. The difference were significant. When the injection of SVC was stopped 10 d later,the difference in the inhibition rates of weight was also significant. The results attest that SVC has inhibitory effects on Eca 109 cells and EAC mice,while SVC has no apparent effects on HeLa cells.

关 键 词：蝎毒 抗肿瘤作用 马氏钳蝎 肿瘤细胞 

分 类 号：R73－361[医药卫生—肿瘤] R282.740.5[医药卫生—临床医学]