人涎腺腺样囊性癌肺转移裸鼠模型的建立  

The Establishment of Lung-Matastasis Model of Human Salivary Adenoid Cystic Carcinoma in Nude Mice

作　　者：关晓峰[1] 邱蔚六[1] 何荣根[1] 林国础[1] 周晓建[1] 

机构地区：[1]上海第二医科大学口腔医学院,中国医科大学口腔系口腔颌面外科

出　　处：《华西口腔医学杂志》1995年第3期147-150,共4页West China Journal of Stomatology

摘　　要：应用经体内外筛选和克隆化的肺高转移性涎腺腺样囊性癌Ａｃｃ－Ｍ细胞株建立肺转移裸小鼠模型。选用３月周龄雌性ＢＡＬＢ／Ｃｎｕ／ｎｕ裸小鼠６０只，经尾静脉接种１×１０６／０．２ｍｌ单细胞悬液。接种后１～３周内，组织学观察可见血管内有癌栓形成；接种后３～４周内为微转移灶期；接种后４～８周为巨视期，肉眼下可见转移灶；接种后８周动物死于肺转移。转移灶体内生长曲线表明，转移灶在４～５周时生长最快；转移灶潜伏期及带瘤生存期均较长，该模型是研究涎腺腺样囊性癌肺转移的良好模型。Abstract In present study authors have established a model for studying SACC lung metastases in nude mice,After the tail vein injection Of 1×106/0.2 ml cell suspension prepared with selected highly lung metastatic salivary adenoid cystic carcinoma(Acc-M),metastatic disease experienced latent phase(1～3W),microscopic metastasis phase(3～4W),and microscopic metaseasis phase(4～8W).The metastases developed the fastest within 4～5W.In macroscopic phase,the metastatic noduli were heterogenous in size, the smallest was 1 mm,the biggest was 6 mm;the number range was 5～18;the noduli located in the peripheral part of the lung lobe.At the end of the 8 th week.the metastatic noduli would mix each other,occupied the whole lung and caused the death of nude mice.

关 键 词：涎腺肿瘤 腺样囊性癌 肺转移瘤 动物模型 

分 类 号：R739.870.2[医药卫生—肿瘤]