RAPD技术在金针菇菌株鉴别的应用  

DISTINGUISHING STRAINS OF FLAMMULINE VELUTIPES WITH RAPD

作　　者：詹才新[1] 朱兰宝[1] 杨新美[1] 

机构地区：[1]武汉华中农业大学植保系,上海市农科院食用菌研究所

出　　处：《华中农业大学学报》1995年第3期253-258,共6页Journal of Huazhong Agricultural University

摘　　要：用２０个随机引物对１６个不同来源的金针菇菌株进行了ＲＡＰＤ分析研究，结果表明：２０个随机引物中，有６个引物的扩增产物ＤＮＡ片段表现出明显的多态性，其中每个引物对不同菌株扩增出现的ＤＮＡ片段数目，少则没有，多达ｌｌ条，平均为５条，ＤＮＡ片段从０．４ｋｂ到３．３８ｋｂ；并且不同的引物扩增出的ＤＮＡ片段带谱不同，差异较大，这６个引物对１６个金针菇菌株共扩增出８４条ＤＮＡ片段带。采用系统聚类法中的类平均法，对１６个菌株两两相似系数聚类分析生成树状图谱，可分为四大类，能直观准确地揭示菌株间的差异，并加以鉴别。因此，ＲＡＰＤ技术是一种快速、准确、有效地鉴别菌株的方法。In this paper,the random amplified polymorphic DNA(RAPD)analysis was conducted with 20 random primers in Various strains of Flammulina velutipes collected fronl various localities. The results revealed that six out of the twenty random primers were scorable polymorphic ones,Scorable DNA fragments derived from each primer amplying in various strains were found to range from zero to eleven with an averge of five. The size of the amplified DNA fragments ranged from 400 to 3380 base pairs。 Of each primer tested,a wide variation in banding profiles among the l6 strains of Flammulina velutipes were observed. In total,eighty-four band positions were scored(l/0)for all of the tested strains.Cluster analysis based on coefficient of genetic similiarity between and among the tested strains of Flammulina velutipes were carried out by UPGMA, All the tested strains were subdivided into four groups through cluster analysis. Therefore, RAPD technique may be ascribed as one of the effective methods to distinguish strains.

关 键 词：金针菇 RAPD 聚类分析 菌株鉴别 

分 类 号：S646.19[农业科学—蔬菜学]