导数同步荧光法测定硒  

Synchronous Scanning Derivative Spectrofluorimetry for the Determination of Selenium with 2,3-Diaminophthalene

作　　者：赵振华[1] 田德海[1] 全文褶 

机构地区：[1]北京市环境保护科学研究院

出　　处：《环境科学》1995年第4期54-56,74,共4页Environmental Science

基　　金：北京市自然科学基金

摘　　要：对硒与２，３－二氨基萘（ＤＡＮ）络合物在有机溶剂中的同步荧光光谱进行了研究。当同步荧光光谱的△λ选择１４０ｎｍ时，可获得－锐形单峰光谱，在此基础上获得了一阶导数同步荧光和二阶导数同步荧光的最佳实验条件。用一阶导数同步荧光定量测定硒可提高方法的灵敏度和改善精密度，在仪器的中挡灵敏范围，硒的检测限为１．５ｎｇ，硒浓度在０—２５０ｎｇ时，硒浓度与荧光强度的直线关系是ｙ＝０．４４ｘ—０．１８，相关系数（ｒ）０．９９８５，１０次测定的变异系数（ＣＶ％）为６．７％。对水样、血液和头发中的硒进行了实测。The synchronous fluorescence spectra of fluorescent chelate of selenium with 2,3-diaminophthalene(DAN) and its synchronous scanning derivative spectra were studied. The specific △λof the synchronous spectra was found to be 140 urn. The first derivative spectrofluorimetry for the determination of selenium with DAN was described. The limits of detection were 1. 5, 1. 9 and 3.0 for the first derivative, second derivative and normal spectrofluorimetric technique, respectively. Coefficient of variation was 6. 7 for the first derivative spectrofluorimetry, and 9.2 for the normal spectrofluorimetry.

关 键 词：硒 二氨基萘 导数同步荧光法 测定 

分 类 号：X132[环境科学与工程—环境科学]