血小板源生长因子及其受体基因表达在低氧大鼠肺中的变化  

Changes of Gene Expression of PDGF and Its Receptor in Hypoxic Rat

作　　者：鲁青 孙仁宇[1] 张宏[1] 余漫 薛全福[1] 

机构地区：[1]中国医学科学院基础医学研究所

出　　处：《基础医学与临床》1995年第3期32-35,共4页Basic and Clinical Medicine

基　　金：国家八五攻关资助

摘　　要：本文观察了血小板源生长因子（ＰＤＧＦ）Ａ、Ｂ链及其α、β受体基因表达在低氧大鼠肺中的变化。Ｎｏｒｔｈｅｒｎ印迹杂交显示正常大鼠肺中可表达ＰＤＧＦ－Ａ链、Ｂ链和ＰＤＧＦ－α受体、β受体的ｍＲＮＡ。随着低氧时间的延长，ｍＲＮＡ水平迅速增加。ＰＤＧＦ－Ａ、Ｂ链ｍＲＮＡ均在低氧的第二天达高峰，分别为正常的１．５１倍及１．６９倍（均为Ｐ＜０．０５），且Ｂ链ｍＲＮＡ基本维持在该水平至第七天。ＰＤＧＦ－α、β受体的ｍＲＮＡ在低氧第四天时达高峰，分别为正常的１．７０倍及１．３４倍（均为Ｐ＜０．０５）。提示ＰＤＧＦ受体及配体基因的依序和／或合并表达增强在低氧性肺血管结构重建中起一定作用。his study examined the changes of platelet-derived Growth Factor(PDGF)receptor and itsligand genes expression in hypoxic rats lungs. Northern blots analysis revealed that normal lungexpresses PDGF-A,-B chain and PDGF-α,-β receptor mRNA. With the longer of hypoxic,thelevels of mRNA increased rapidly, The mRNA of PDGF-A,and B chain reached a maximum atday 2,respectively 1. 51 fold and 1.69 fold as compared with control,(P<0.05 ),and B chainsustained at this high level to day 7.The mRNA of PDGF-α and-β receptor reached a maximumt day 4,respectively 1.70 fold and 1.34 fold as compared with control(P<0.05). Results suggested that increases of PDGF receptor and its ligand genes expression may becoordinately and subsequently involved in hypoxic pulmonary vascular remodeling.

关 键 词：血小板源生长因子 低氧 基因表达 

分 类 号：R364.4[医药卫生—病理学]