乳腺癌血清蛋白质组与尿代谢物组协同分析研究  

Studies on integrated analysis of serum proteomics and urinary metabonomics of breast cancer patients

作　　者：沈朋[1] 曾真[2] 程翼宇[2] 

机构地区：[1]浙江大学医学院附属第一医院,杭州310003 [2]浙江大学药物信息学研究所

出　　处：《中华检验医学杂志》2005年第7期710-712,共3页Chinese Journal of Laboratory Medicine

基　　金：国家自然科学基金重大研究计划重点资助项目(90209005);浙江省科技计划项目(2004C33026)

摘　　要：目的研究血清蛋白质组和尿代谢物组协同分析乳腺癌诊断方法。方法用双向电泳、高效液相色谱和液质联用技术对7份正常人与14份乳腺癌患者血清蛋白质组和尿代谢物组进行分析,从中寻找差异蛋白和代谢组模式差异。结果与正常人相比,乳腺癌患者血清中谷胱甘肽S转移酶M5呈高表达状态,尿中乳清酸核苷、1甲酰化腺苷、S腺苷L蛋氨酸及N2甲酰化鸟苷等4种核苷代谢物组成模式异常。结论乳腺癌患者体液内化学物质与正常人相比具有特异性模式差异。经肿瘤蛋白质组与代谢物组协同研究,有望建立肿瘤早期诊断新方法,进行肿瘤发病机理研究。Objective To study on the diagnosis methodology of breast cancer using integrated analysis of proteomics and metabonomics approach. Methods The serum samples from the 14 breast cancer patients and 7 healthy people were analyzed by two-dimensional electrophoresis in order, to find different ialexpressed proteins. On the other hand, an HPLC-based urinary metabonomics profiling was employed to pick out chemical patterns between breast cancer patients and healthy people. Results Glutathione S-transferase M5 was found highly expressed in the breast cancer patients＇ serum. The concentration pattern of SAdenosylmethionine, Orotidine, 1-Methyladenosine and NZ-Methylguanosine in patients＇ urine were peculiarly different from that in the healthy people. Conclusion There are peculiar chemical pattern differentiae between the body fluid of breast cancer patients and the body fluid of healthy people. Synthetic study of cancer proteomics and metabonomics can contribute not only to prognosis of cancer but also to the pathogenesis of cancer.

关 键 词：乳腺癌 血清蛋白质组 尿代谢物组 协同分析 差异蛋白 

分 类 号：R737.9[医药卫生—肿瘤] R730.43[医药卫生—临床医学]