膀胱肿瘤中错配修复基因hMLH1启动子甲基化状态的研究  

Study of promoter methylation of mismatch repair gene hMLH1 in bladder neoplasms

作　　者：赵敏[1] 李智[1] 于永春[2] 段建敏[3] 许云飞[2] 

机构地区：[1]同济大学附属第十人民医院检验科,上海200072 [2]同济大学附属第十人民医院中心实验室,上海200072 [3]兰州医学院第二附属医院泌尿外科

出　　处：《中华检验医学杂志》2005年第7期736-738,共3页Chinese Journal of Laboratory Medicine

摘　　要：目的探讨膀胱肿瘤中错配修复基因hMLH1启动子甲基化状态及其意义。方法收集30对新鲜的膀胱癌组织及其癌周正常组织;采用甲基化特异聚合酶链反应(methylationspecificPCR,MSP)检测hMLH1基因启动子区CpG岛的甲基化状态。结果30对膀胱癌组织、癌周正常组织中hMLH1基因的甲基化阳性率分别为10/30、1/30,二者差异有统计学意义(P<0.01);hMLH1基因甲基化阳性率在Ta～T1期、T2～T3期膀胱癌中分别为7/19、3/11,二者差异无统计学意义(P>0.05);在Ⅰ、Ⅱ、Ⅲ级膀胱癌中分别为7/18、2/9、1/3,三者差异无统计学意义(P>0.05)。结论错配修复基因hMLH1在膀胱癌组织中有一定程度的过甲基化,但与膀胱癌的临床病理特点无显著相关性,提示hMLH1的异常甲基化可能参与了膀胱肿瘤的发生。Objective To explore the methylation status of mismatch repair gene hMLH1 in bladder cancer and its meaning. Methods Thirty fresh bladder tumor tissues and paired adjacent normal tissues were obtained from cystectomy specimens. The CpG island methylation status of hMLH1 was examined by means of methylation specific PCR （MSP）. Results hMLH1 methylation was present in 10 of 30 tumor tissues and in 1 of 30 normal tissues ; there was significant difference between them（ P 〈 0.01 ）. The positive rates of hMLH1 methylation in stage Ta - T1 and T2 - T3 bladder cancer were 7 of 19 and 3 of 11,respectively; there was no significant difference between them （P 〉 0.05 ）. The positive rates in grade Ⅰ,Ⅱ and Ⅲ bladder cancer were 7 of 18, 2 of 9 and 1 of 3, respectively; there was no significant difference among them（ P 〉 0. 05 ）. Conclusion There exists a certain degree of hMLH1 methylation in bladder cancer, but it does not correlate with stage and grade of bladder cancer, suggesting that the aberrant methylation of hMLH1 may be involved in the tumorigenesis of bladder neoplasams.

关 键 词：膀胱肿瘤 错配修复基因 HMLH1 基因启动子 基因甲基化 

分 类 号：R737.14[医药卫生—肿瘤]