戊唑醇、丙环唑和三唑酮的GC和HPLC分析方法  

Study on the Quantitative Analysis of Tebuconazole,Propiconazole and Triadimefon by GC and HPLC

作　　者：慕卫[1] 刘峰[1] 孙作洋[1] 魏光[1] 杨连来[1] 

机构地区：[1]山东农业大学植物保护学院,山东泰安271018

出　　处：《农药科学与管理》2005年第7期1-3,共3页Pesticide Science and Administration

摘　　要：采用气相色谱法和液相色谱法2种方法分别对戊唑醇、丙环唑和三唑酮进行定性定量分析,气相色谱法中都以邻苯二甲酸二戊酯为内标物,色谱柱都采用3%OV-101chromosorbAWDMCS150￣177μm1m×3mm玻璃柱,柱温为200～205℃,汽化温度和检测温度均为230℃,戊唑醇、丙环唑和三唑酮的回收率分别为99.1%～100.3%,98.5%～99.8%和99.3%￣100.7%;方法变异系数分别为0.34%,0.51%和0.37%。高效液相色谱法中使用Nova-PaKC18250mm×4.6mm色谱柱,以甲醇+水=85+15为流动相,检测波长为225nm。戊唑醇、丙环唑和三唑酮的回收率分别为99.1%～100.7%,98.9%￣99.6%和99.9%～101.2%;方法变异系数分别为0.74%,0.43%和0.58%。The quantitative analysis of tebuconazole, propiconazole and triadimefon by GC and HPLC were studied.The operation conditions of gas chromatographic method were： 3%OV-101 on Chromosorb AW DMCS, 1m×3mm （i.d.） glass column at column temperature of 200-205℃ and detector temperature of 230℃.The internal standard was disoamyl phthalate.The recoveries were 99.1%-100.3%, 98.5%-99.8% and 99.3%-100.7%, their coefficients of variation were 0.34%,0.51% and 0.37% respectively. The operation conditions of HPLC were： C18 column （250mm×4.6mm） , methanol ＋water=85 ＋15 （V/V） as mobile phase, UV detector at 225 nm.The recoveries of this method were 99.1%-100.7%, 98.9%-99.6% and 99.9%-101.2%.The coefficients of variation were 0.74% , 0.43% and 0.58% for tebuconazole, propiconazole and triadimefon respectively.

关 键 词：GC HPLC 分析 戊唑醇 丙环唑 三唑酮 

分 类 号：S482.2[农业科学—农药学] O657.71[农业科学—植物保护]