猪血超氧化物歧化酶、血红素和蛋白胨分离制备的研究  

STUDY OF EXTRACTION AND PREPARATION OF SUPEROXIDE DISMUTASE,HEMATIN AND PEPTONE FROM HOG BLOOD

作　　者：凌敏[1] 蔡丹昭[1] 苏上贵[1] 黄勇奇[1] 赖祥进[1] 

机构地区：[1]广西医科大学生物化学与分子生物学教研室,南宁530021

出　　处：《广西医科大学学报》2005年第3期363-364,共2页Journal of Guangxi Medical University

基　　金：广西自然科学基金资助(No.桂科自9912035)

摘　　要：目的:研究从猪血分离制备超氧化物歧化酶(SOD)、血红素(Hematin)和蛋白胨(peptone)的工艺流程。方法:SOD的分离纯化采用加热变性、盐析和凝胶过滤的方法;血红素的提取采用鞣酸法;蛋白胨的制备采用碱性酶解法。结果:根据这一工艺流程,能从1000ml猪血的红细胞中获得总活力为25.8×104U、比活力为1.19×104U/mg的SOD和产量达2678mg、纯度达92.3%的血红素,并且还从猪血总蛋白中制备了含Arg、Leu、Lys、Phe、Val丰富的蛋白胨。结论:从猪血中成功制备了产率与纯度较高的SOD、血红素以及营养价值较高的蛋白胨。Objective： To study the technological procedure of extraction and preparation of superoxide dismutase （SOD）, hematin and peptone from hog blood. Methods ：Heating denaturation, salting out and gel filtration chromatography were used to extract SOD;tannic acid method was used to purify hematin;the method of basic hydrolysis with protease was applied to produce peptone. Result： By this procedure we obtained purified SOD possessing a total activity of 25.8× 10^4U and specific activity of 1.19× 10^4 U/rag, purified hematin of 2 678 mg and 92.3% purity from hog red cells of 1 000 ml blood as well as peptone with plenty of Arg,Leu,Lys,Phe,Val from total protein of hog blood. Conclusion：Purified SOD, hematin and nourishing peptone were successfully extracted and prepared from hog blood.

关 键 词：猪血 超氧化物歧化酶 血红素 蛋白胨 

分 类 号：Q51[生物学—生物化学]