牛体外受精胚的发育速度和染色体研究  

Developmental speed of in vitro fertilized bovine embryosand analyses of their chromosomes

作　　者：唐峰[1] 朱淑文[1] 华修国[1] 张菁[1] 朱秀萍[1] 

机构地区：[1]上海交通大学农业与生物学院,上海201101

出　　处：《畜牧与兽医》2005年第5期7-9,共3页Animal Husbandry & Veterinary Medicine

基　　金：上海市科技兴农重点攻关项目[农科攻字(2002)第1-1-2-2号]

摘　　要：利用不同个体的牛冷冻精液对屠宰场废弃牛卵巢内卵母细胞进行体外受精,对体外受精胚胎的发育速度和染色体的异常发生进行了研究。结果显示,冷冻精液A组体外受精48h后的卵裂率显著高于冷冻精液B组(P<0.05),2组之间的囊胚形成率差异显著(P<0.05);冷冻精液A体外受精后的胚胎发育速度显著快于冷冻精液B组(P<0.05);染色体分析结果表明,2组胚胎染色体异常发生率无显著差异,异常胚胎均表现为多倍体的发生率较高。The frozen-thawed semen from different bulls were used for in vitro fertilization （IVF） with bovine oocytes to study in vitro development speed of IVF bovine embryos and the occurrence of the chromosome aberration. The results showed that at 48h after IVF, the cleavage rate in frozen-thawed semen group A was significantly higher than that in group B （ P 〈 0. 05 ） , and the rates of blastocysts were also different （ P 〈 0. 05）. The speed of IVF embryo development in group A was increased contrary to that of group B （ P 〈 0. 05 ）. According to chromosomal analyses, there was no significant difference between the two groups in the rate of chromosomal abnormalities and the high rate of polyploid was observed in both groups.

关 键 词：冷冻精液 卵裂率 囊胚形成率 染色体异常 牛 

分 类 号：S823.3[农业科学—畜牧学]