改良法重建肝动脉血供的大鼠原位肝移植模型  

Modified orthotopic liver transplantation with hepatic artery reconstruction in rats

作　　者：陈志宇[1] 张玉君[1] 王槐志[1] 董家鸿[1] 

机构地区：[1]第三军医大学西南医院全军肝胆外科研究所解放军西南肝胆外科医院,重庆400038

出　　处：《中华实验外科杂志》2005年第8期1016-1017,共2页Chinese Journal of Experimental Surgery

摘　　要：目的建立一种稳定的重建肝动脉血供的大鼠原位肝移植模型,为研究肝移植术后移植免疫、胆道并发症等提供一个更加符合生理的动物模型。方法以经典的“双袖套”大鼠原位肝移植模型为基础,将带有主动脉的供肝动脉与受体腹主动脉行端侧吻合重建肝动脉血供。结果共施行重建肝动脉血供的大鼠原位肝移植35例。术后24h动物存活率85.7%,术后1周动物存活率82.9%。结论用带有主动脉的供肝动脉与受体腹主动脉行端侧吻合重建肝动脉血供的方法稳定可靠,易于标准化。该模型是研究肝移植术后免疫排斥、胆道并发症的理想模型。Objective To establish a stable orthotopic liver transplantation model with hepatic artery reconstruction in rats and to provide a more physiological way to investigate transplantation immunology and hiliary complications after liver transplantation. Methods On the hasis of classic “two cuff” orthotopic liver transplantation in rats, graft aorta end-to-side anastomosis to recipient aorta was applied to reconstruct hepatic artery. Results Liver transplantation with hepatic artery reconstruction was conducted in 35 rats. The 24-h and one-week survival rate after operation was 85.7 % and 82.9 %, respectivily. Conclusion This method （graft aorta end-to-side anastomosis to recipient aorta） is stable and easy to be standardized. This model is ideal in the study of immune rejection and biliary complications.

关 键 词：肝动脉血供 大鼠 原位肝移植 动物模型 免疫排斥 

分 类 号：R-332[医药卫生]