纳洛酮对东莨菪碱所致大鼠空间工作记忆障碍的干预效应(英文)  

Intervention effect of naloxone on scopolamine-induced impairment of spatial working memory in rats

作　　者：段新 马光瑜 张艳美[3] 

机构地区：[1]佛山市顺德区伍仲纪念医院老年科,广东省佛山市528333 [2]广东省精神卫生研究所,广东省广州市510180 [3]汕头大学医学院电镜室,广东省汕头市515041

出　　处：《中国临床康复》2005年第20期248-251,i0002,共5页Chinese Journal of Clinical Rehabilitation

基　　金：广东省自然科学基金资助项目(000825)~~

摘　　要：背景:隔-海马区的胆碱能神经通路是空间学习记忆的重要部位,此区神经元中又富含阿片受体。有研究表明阿片类受体拮抗剂纳洛酮能够解除内侧隔区域及海马部位阿片肽对胆碱能神经元活性的抑制,促进突触间隙乙酰胆碱释放,改善空间记忆损害。目的:观察阿片类受体拮抗剂纳洛酮对M胆碱能受体阻滞剂东莨菪碱所致大鼠空间工作记忆障碍的干预作用。设计:以实验动物为观察对象的随机对照实验。单位:佛山市顺德区伍仲纪念医院老年科。材料:实验于2003-04在汕头大学医学院动物实验中心完成。清洁级SD大鼠21只,随机分成3组,正常组、东莨菪碱组和纳洛酮治疗组(东莨菪碱+纳洛酮),每组7只。自制水迷宫为圆形橡胶水池,直径2m,高55cm,水深30cm,水温20～22℃,在水池东南象限中央放置一圆柱形平台,平台直径12cm,距水面1.5cm,此平台为大鼠唯一可逃生之地。池水表面均匀覆以白色聚乙烯碎屑,房内设施如灯管、窗户及桌子等固定,可作为水池外参照目标,水池四壁任意挂置醒目标志数个,可作池内参照目标。方法:各组大鼠均于实验前30min腹腔给药,东莨菪碱组(0.4mg/kg),纳洛酮治疗组(3mg/kg),正常对照组参照东莨菪碱组剂量予等体积生理盐水。然后采用自制水迷宫延缓性匹配任务试验记录每只大鼠前后两次实验逃避潜伏期的时间。于水迷宫测试后迅即处死大鼠,取其左侧大脑组织,常规处理切片。定量测定各组大鼠脑海马和前额叶皮质胆碱乙酰转移酶的表达,采用免疫组化及图像分析技术。同时各组大鼠2只取右侧大脑组织,迅速游离海马,观察大鼠脑海马CA1区的改变,采用电子显微镜技术。所有数据用x±s表示,每组延缓性匹配作业前后两次逃避潜伏期比较采用配对资料t检验;各组间均数比较采用方差分析。以P<0.05为显著性差异。主要观察指标:①各组大鼠前后两次逃�BACKGROUND： The pathway of cholinergic nerve also with rich opium receptor in the septum-hippoeampus areas is an important part for spatial learning memory. There is study that naloxone, an antagonist of opium receptor, can relieve the inhibition of activity of cholinergic neuron caused by opioid peptides in the medial septum and hippocampus, promote the release of acetylcholine （Ach）into synaptic cleft, and improve the impairment of spatial memory.OBJECTIVE： To investigate the intervention effect of naloxone on impairment of spatial working memory of rats induced by scopolamine,a blocker of cholinergic muscarinic receptor.DESIGN： A randomized and controlled trial taking experimental animals as subjects.SETTING： Geriatric Department of Wuzhongpei Memorial Hospital at Shunde District of Foshan City.MATERIALS： The experiment was completed from 2003 to 2004 at the Experimental Animal Center of Medical School of Shantou University. A total of 21 SD rats of clean grade were at random divided into three groups：normal group, scopolamine group and naloxone group （scopolamine and naloxone）, with 7 in each group. Build up of Morris water maze： A circular rubber pool, 2 m diameter and 55 cm height, with 30 cm depth of water at 20℃-22℃, was built up. At the center of its southeast quadrant, a cylinder platform installed, which was 12 cm in diameter and 1.5 cm below the water surface, the only place for rats to escape death. The water of the pool was covered with bits of white polythene. In the house, there were light tubes, windows and tables in fixed place served as the outside cues of the pool. On the four walls of the pool, there were several randomly-hung and eye-catching markers as inside cues.METHODS： Medicines were intraperitoneally given 30 minutes before the experiment for rats in each group, scopolamine 0.4 mg/kg and naloxone 3mg/kg in two treatment groups, the equal volume saline in normal group.Then escape latencies of rats were recorded for two successive trials through a delayed-matc

关 键 词：记忆障碍 东莨菪碱 纳洛酮 大鼠 空间工作记忆障碍 清洁级SD大鼠 海马CA1区超微结构 干预效应 胆碱能受体阻滞剂 阿片类受体拮抗剂 

分 类 号：R749[医药卫生—神经病学与精神病学] R-332[医药卫生—临床医学]