13q14断裂重排与非小细胞肺癌转移潜能关系的研究  

13q14 Aberration is Related to the Metastatic Potential of Human NSCLC

作　　者：黄昀[1] 杨焕杰[1] 金焰[1] 李慧敏[1] 傅松滨[1] 

机构地区：[1]哈尔滨医科大学遗传学研究室,哈尔滨150086

出　　处：《遗传》2005年第4期531-534,共4页Hereditas（Beijing)

基　　金：国家自然科学基金(编号:30370783);高等学校优秀青年教师科研奖励计划;黑龙江省科学技术攻关项目(编号:GB03C6011)~~

摘　　要：发生转移的肿瘤细胞经常存在染色体数目异常和结构畸变,在多种有转移潜能的肿瘤细胞中都涉及到13q14的异常。以往研究表明,在同一组织来源但转移潜能不同的肺腺癌细胞系AGZY83a和Anip973中存在13q14的断裂重排。采用mRNA差异展示技术(mRNADD)分析这一对细胞系得到的差异表达基因BRI基因位于13q14。为了进一步分析肺癌细胞的转移潜能与13q14断裂重排间的关系,采用13q涂染探针对具有不同转移潜能的非小细胞肺癌细胞系PAa、SPC1A和95D中期分裂相进行G显带后的荧光原位杂交分析。结果发现,在3个肺癌细胞系中有多种13号染色体长臂的结构异常,其中此3个细胞系均涉及13q3233的频发断裂。但是低转移肺癌细胞系PAa、SPC1A均未涉及13q14的断裂,而高转移肺癌细胞系95D的两种细胞克隆均可见13q14的断裂。提示13q14断裂点与肺癌细胞的转移能力有一定的相关性,两者之间的遗传学意义需要进一步研究探索。A large number of numerical and structural aberrations were analyzed in human tumor metastatic cells and 13q14 aberrations were frequently detected in some types of metastatic cancers. The rearrangement of 13q14 was identified previously in two lung adenocarcinoma cell lines with the same origin but different metastatic potential AGZY83-a and Anip973. BRI gene showed different expression levels in the cell lines as revealed by mRNA differential display （mRNA DD） in the two cell lines, and located in 13q14. In order to investigate the relationship between 13q14 abnormalities and tumor metastasis, a painting probe （13q） was used to hybridize three G-banded NSCLC cell lines with different metastatic potential. The major abnormality of 13q differs among different cell lines, including 13q32-33 frequent breakpoint in these three cell lines. But low metastatic potential cell lines PAa, SPC-｜-A were not found breakpoint in 13q14, while 95D cell line with high metastatic potential had the common breakpoint 13q14 in two cell clones, The results suggested that the breakage at 13q14 may possibly be related to lung cancer metastasis. The affirmative relationship between 13q14 aberration and NSCLC needs further investigation.

关 键 词：非小细胞肺癌 染色体重排 转移 染色体涂染 荧光原位杂交 

分 类 号：R734.2[医药卫生—肿瘤]