乳宁Ⅱ号方对小鼠Ca761乳腺癌移植瘤细胞周期及p53和ras表达的影响  

Effects of Runing RecipeⅡ on expressions of p53 and ras oncogene proteins and cell cycle of the transplanted Ca761 breast cancer in mice

作　　者：陈前军[1] 王一安[1] 司徒红林[1] 陆德铭[2] 任黎萍[1] 赖熙雯[1] 刘鹏熙[1] 林毅[1] 

机构地区：[1]广州中医药大学广东省中医院乳腺科,广东广州510120 [2]上海中医药大学龙华医院乳腺科,上海200032

出　　处：《中西医结合学报》2005年第3期225-228,共4页Journal of Chinese Integrative Medicine

摘　　要：目的:通过研究乳宁Ⅱ号方对小鼠Ca761乳腺癌移植瘤细胞周期及p53、ras表达的影响,探讨乳宁Ⅱ号方抑制乳腺癌生长的机制。方法:建立小鼠Ca761 乳腺癌移植模型,小鼠造模后随机分成生理盐水对照组、环磷酰胺(cyclophosphamide, CTX)治疗组、乳宁Ⅱ号方治疗组及乳宁Ⅱ号方加CTX 治疗组,每组12只。用流式细胞术检测经不同方法处理的荷瘤小鼠移植瘤细胞的细胞周期,同时运用免疫组化法测定移植瘤组织中p53和ras癌基因蛋白的表达,并进行组间比较。结果:乳宁Ⅱ号方治疗组、CTX治疗组与乳宁Ⅱ号方加CTX治疗组S期肿瘤细胞与生理盐水对照组比较明显减少(P<0.05);乳宁Ⅱ号方治疗组G0 G1期肿瘤细胞百分比低于CTX治疗组(P<0.05),而G2 M期细胞百分比高于CTX治疗组(P<0.05);乳宁Ⅱ号方治疗组与乳宁Ⅱ号方加CTX治疗组p53的表达均显著低于生理盐水对照组(P<0.05),而CTX对p53表达的影响不显著(P>0.05);乳宁Ⅱ号方治疗组、CTX治疗组与乳宁Ⅱ号方加CTX治疗组ras的表达均低于生理盐水对照组(P<0.05)。结论:乳宁Ⅱ号方可通过调控小鼠Ca761 乳腺癌移植瘤细胞的细胞周期来影响肿瘤的生长,这一作用与其调控肿瘤组织p53和ras基因表达有关。Objective. To explore the mechanisms of Runing Recipe Ⅱ （a recipe composed of traditional Chinese herbs） in inhibiting the growth of breast cancer by observing its effects on the expressions of p53 and ras oncogene proteins and cell cycle of the transplanted Oa761 breast cancer in mice. Methods： We established the breast cancer model by transplanting Ca761 cells in mice. The mice were randomly divided into 4 groups： normal saline control group, CTX-treated group, Runing Recipe Ⅱ-treated group, and RuningRecipe Ⅱ and CTX-treated group, with 12 mice in each group. We detected the cell cycle of the cancer cells in the mice＇s transplanted tumor with flow cytometry and measured the expressions of p53 and ras oncogene proteins in the transplanted tumor with immunohistochemical methods. Results： The percentages of tumor cells in S-phase of the Runing Recipe Ⅱ-treated group, CTX-treated group and Runing Recipe Ⅱ and CTX-treated group were significantly lower than that of the normal saline control group respectively （P＜0.05）.The percentage of tumor cells in G0-G1 phase of the Runing Recipe Ⅱ -treated group was lower than that of the CTX-treated group （ P＜0.05）, while the percentage of tumor cells in G2-M phase was higher than that of the CTX-treated group. The immunoreactive scores （IRSs） of p53 in the Runing Recipe Ⅱ-treated group and Runing Recipe Ⅱ and CTX-treated group were significantly lower than that in the normal saline control group respectively （P＜0.05）. The effect of CTX on the expression of p53 was not significant. The IRSs of ras oncogene protein in the Recipe Ⅱ-treated group, CTX-treated group and Runing Recipe Ⅱ and CTX-treated group were lower than that in the normal saline control group respectively （P＜0.05）. Conclusion： Runing Recipe Ⅱ can inhibit the growth of Ca761 breast cancer in mice by controlling the cell cycle of the transplanted tumor. This may be related to its effect on the gene expressions of p53 and ras in the tumor tissue.

关 键 词：中国医药学 乳腺癌 细胞分化 癌基因蛋白类 

分 类 号：R737.9[医药卫生—肿瘤]