Fas／FasL、FAP-1在肝母细胞瘤中的表达研究及其临床意义  

Study of Fas、FasL and FAP- 1 Protein Expression in Hepatoblastoma and Its Clinical Significance

作　　者：吴茂军[1] 陈民[2] 田茂良[1] 韩德山[1] 吕方启[1] 

机构地区：[1]山东省泰山医学院附属医院小儿外科,泰安市271000 [2]山东省泰安市肿瘤医院,271000

出　　处：《中华腹部疾病杂志》2005年第9期625-628,共4页Chinese Journal of Celiopathy

摘　　要：目的 探讨肝母细胞瘤组织Fas、FasL及FAP-1和组织中淋巴细胞的Fas、FasL的表达状况及临床意义。方法 应用Fas、FasL多克隆抗体及FAP-1单克隆抗体对15例小儿肝母细胞瘤组织标本进行免疫组化染色，以检测其组织Fas、FasL及FAP-1和淋巴细胞的Fas、FasL的蛋白表达，并以相应的正常肝组织作对照。结果 肝母细胞瘤组织中Fas表达（78％）高于正常肝组织（5％），阳性信号主要位于细胞膜和细胞浆，呈胞浆型表达。正常肝组织中FasL表达阴性，而肝母细胞瘤中FasL表达73％，两者表达率差异明显；在15例肝母细胞瘤中FAP-1阳性表达13例。同时发现瘤周淋巴细胞Fas、FasL的阳性表达率分别为80％、68％，而在正常肝组织中的表达均为阴性。结论 正常肝组织同时表达Fas／FasL，在维持其自身细胞的增殖与凋亡的平衡中起重要作用；肝母细胞瘤组织Fas、FasL表达上调以及FAP-1阳性表达可诱导表达Fas的淋巴细胞凋亡以实现免疫逃逸。AIM To investigate the expression of Fas,FasL and FAP- 1 protein in hepatoblastoma and the xpression of Fas,FasL in lymphocyte and its clinical significance. METHODS The expression of Fas,FasL and FAP- 1 was examined in 15 cases of hepatoblastoma and in 15cases corresponding normal liver tissue with Fas,FasL and FAP - 1 clonal antibody respectively ,by immunohistochemical technique. RESULTS Fas expression rate was higher in hepatoblastoma（78% ）than that in normal liver tissue（5% ）, the staining signal of Fas was mainly located in cytoplasm and membrance. FasL expression in normal liver tissue was negative, but, it is 73 % in hepatoblastoma, and the imparity of expression rate between them is very obvious. That the expression of FAP - 1 was found in all hepatoblastoma is 15 cases. And in this test, we all sofound theexpression rate of Fas,FasLof lymphocyte 80% ,68%, they are negative in normalliver tissue. CONCLUSIONS Coexpression of Fas/FasL play an important role in the balance of proliferation and death in normal liver tissue. The expression of Fas and FasL rise and the expression of FAP- 1 in hepatoblastomacolorectal cancer escapes immune survillance.

关 键 词：肝母细胞瘤 FAS/FASL FAP-1 蛋白表达 免疫逃逸 

分 类 号：R732.23[医药卫生—肿瘤]