以壳聚糖为载体疏水色谱填料的制备及其应用  

Preliminary Study on Chitosan as a Hydrophobic Interaction Chromatographic Matrix

作　　者：王云[1] 郭敏亮[1] 姜涌明[1] 

机构地区：[1]扬州大学生物科学与技术学院

出　　处：《安徽农业科学》2005年第7期1357-1358,共2页Journal of Anhui Agricultural Sciences

摘　　要：以戊二醛交联后的壳聚糖为载体,以正戊醛为配基,通过希夫氏(Schiff-baseformation)制备了正戊基壳聚糖(pentyl-ChitosanCL)疏水色谱填料,并以它装柱对鸡卵类粘蛋白(chickenovomucoid,CHOM)成功地进行了分离纯化,得到了2个胰蛋白酶活性峰,其活性分别为1265和1590U/mg.Pr。The n-valderaldehyde modified chitosan （pentyl-Chitosan CL） was prepared by Schiff-base formation and hydrogenation. Chicken ovomucoid was successfully purified by hydrophobic interaction chromatography using pentyl-Chitosan CL as matrix, the purification fold reached 2.0.

关 键 词：壳聚糖 疏水色谱 吸附 鸡卵类粘蛋白 

分 类 号：Q629.1[生物学—生物物理学]