Fluorescence Determination of Artemisinin Using Tyrosinase as Catalyst and Pyronine B as Monitor  

作　　者：陈莉华 尹红 杨朝霞 张克梅 刘六战 沈含熙 

机构地区：[1]Chemical School, Jishou University, Jishou, Hunan 416000, China [2]Chemical School, Nankai University, Tianjin 300071, China

出　　处：《Chinese Journal of Chemistry》2005年第8期1047-1052,共6页中国化学（英文版）

基　　金：Project supported by the National Natural Science Foundation of China （No. 20075012） and the Natural Science Foundation of Hunan Educational Committee （No. 02C313）.

摘　　要：Fluorescence decrease ratio （F0/F） was applied to determination of artemisinin （qinghaosu, QHS） based on the catalytic effect of tyrosinase using tetraethyldiaminoxanthenyl chloride （pyronine B, PB） as monitor. A catalyst used commonly in the decomposition of QHS, tyrosinase, exhibited higher binding activity than hemin, which was expressed as Michaelis-Menten parameters, km, Vmax, and kcat respectively. Interaction of QHS with tyrosinase was inhibited in the presence of deactivating agents at high temperature whereas enhanced by ethanol. Under optimal conditions, a concentration of 1.4×10^-7-8.4×10^-7 mol·L^-1of QHS could be determined on the basis of fluorescence decrease ratio of PB, with a detection limit 3tr of 2.6×10^-9 mol·L^-1. The proposed method was applied to detection of the concentration of QHS in the media of plasma and urine.Fluorescence decrease ratio （F0/F） was applied to determination of artemisinin （qinghaosu, QHS） based on the catalytic effect of tyrosinase using tetraethyldiaminoxanthenyl chloride （pyronine B, PB） as monitor. A catalyst used commonly in the decomposition of QHS, tyrosinase, exhibited higher binding activity than hemin, which was expressed as Michaelis-Menten parameters, km, Vmax, and kcat respectively. Interaction of QHS with tyrosinase was inhibited in the presence of deactivating agents at high temperature whereas enhanced by ethanol. Under optimal conditions, a concentration of 1.4×10^-7-8.4×10^-7 mol·L^-1of QHS could be determined on the basis of fluorescence decrease ratio of PB, with a detection limit 3tr of 2.6×10^-9 mol·L^-1. The proposed method was applied to detection of the concentration of QHS in the media of plasma and urine.

关 键 词：TYROSINASE ARTEMISININ pyronine B fluorescence decrease ratio 

分 类 号：R978.61[医药卫生—药品]