核因子-κB在角膜移植排斥反应中作用的初步实验研究  

Preliminary experimental study of nuclear factor-κB in the rejection of rat corneal transplantation

作　　者：郑鲜娜[1] 余洪华[1] 

机构地区：[1]广州军区广州总医院眼科,510010

出　　处：《实用医学杂志》2005年第16期1748-1750,共3页The Journal of Practical Medicine

摘　　要：目的:研究核因子-κB在角膜移植排斥反应中的作用,为进一步研究角膜移植排斥反应发病机制及治疗提供新的思路。方法:建立大鼠穿透性角膜移植动物模型,用免疫组织化学染色法检测角膜移植术后植片中核因子-κB表达,并以植片排斥反应指数(RI)及病理学表现作参照。结果:同基因移植组角膜植片中的角膜基质细胞及内皮细胞有核因子-κB的微弱表达;同种异体移植组角膜上皮细胞、内皮细胞、基质层细胞及新生血管内皮细胞核因子-κB表达增强。各组间核因子-κB的表达强度差异有显著性(P<0.01)。且核因子-κB的表达强度与排斥反应指数(RI)具有明显的正相关性(r=0.883,P<0.01)。结论:核因子-κB可能通过调控免疫和炎症相关基因的表达,参与角膜移植排斥反应的发生,在角膜移植免疫中起着中心调控者的作用。Objective To investigate the expression of NF-κB in corneal graft rejective reaction in rats. Methods The model of orthotopical corneal transplantation was developed in rats. The rats were divided into two groups： syngeneic control （Wistar to Wistar） and allograft group （Wistar to SD）. Immunohistochemistry staining was used to analyze NF-κB expression of corneal graft in each group. Results NF-κB was weakly expressed on keratocytes, corneal endothelial ceils and not expressed on corneal endothelial ceils after transplantation in syngeneic group. NF-κB was obviously expressed on corneal epithelial cells, keratocytes, corneal and vascular endothelial ceils in ailograft group. The difference of NF-κB expression between two groups was significant（P〈0.01）. Clinical observation showed the expression level of NF-κB was significantly correlated with rejection index（RI） （r=0. 883, P 〈0.01） . Conclusion NF-κB may regulate the expression of genes associated with graft rejection, and thus play a role of center-control in the occurance and development of corneal graft rejective reaction.

关 键 词：核因子-ΚB 角膜移植 排斥反应 实验研究 发病机制 

分 类 号：R779.65[医药卫生—眼科]