TNF相关凋亡诱导配体对肝癌细胞系生物学活性的影响  

Effect of TNF-related apoptosis inducing ligand on the biological activity of hepatocarcinoma cell line

作　　者：韩丽辉[1] 孙汶生[1] 刘素侠[1] 贾晓青[2] 王晓燕[1] 马春红[1] 高立芬[1] 张利宁[1] 曹英林[1] 

机构地区：[1]山东大学医学院,山东济南250012 [2]山东大学齐鲁医院,山东济南250012

出　　处：《中国病理生理杂志》2005年第8期1486-1490,共5页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No.30371342;No.30128023);山东省基金资助项目(No.2004BS03010)

摘　　要：目的:探讨新型凋亡分子TNF相关凋亡诱导配体(TRAIL)对人肝癌细胞系的作用及生物学活性的影响。方法:免疫细胞化学法检测HepG2细胞表面膜结合型TRAIL的表达,ELISA法检测HepG2细胞分泌型TRAIL的表达。MTT和TUNEL法分别进行细胞毒实验和凋亡的检测。HepG2细胞内端粒酶的活性由TRAP-PCR法检测,端粒酶催化亚单位hTERT的表达由流式细胞术进行检测。结果:HepG2肝癌细胞系表面组成性表达TRAIL分子,并有适量的sTRAIL呈分泌表达。细胞毒实验显示TRAIL能够显著抑制肝癌细胞的生长,TUNEL检测结果显示TRAIL具有诱导肝癌细胞凋亡的效应。端粒酶的活性检测显示,TRAIL能够有效抑制肝癌细胞系内端粒酶的活性以及端粒酶催化亚单位的表达。结论:TRAIL是一个有效的抑癌分子,能够通过诱导细胞凋亡、抑制端粒酶活性等途径控制肿瘤细胞的生长和进一步杀伤肿瘤细胞。AIM： To explore the effect of TNF- related apoptosis inducing ligand （TRAIL）, a new apoptotic inducin gmolecule on the biological activity of hepatocarcinoma cell line. METHODS： The expression of membrane binding TRAIL on HepG2 cells was detected by immuno - cytechemistry. Quantity of secretory TRAIL was assayed by ELISA method. The cytotoxicity and apoptosis induced by TRAIL was detected by MTr and TUNEL method, respectively. The telomerase activity of HepG2 cells was detected by TRAP - PCR assay kit. The expression of hTERT, the catalytic subtmit of telomerase, was detected by FCM. RESULTS： TRAIL was constitutively expressed on the membrane of HepG2 cell line. Soluble TRAIL was also expressed to a certain degree. Cytotoxicity assay showed that TRAIL significantly inhibited the growth of hepatocarcinoma cells. TUNEL assay indicated that TRAIL induced apoptosis in hepatocarcinoma ceils. Detection of telomerase activity showed that TRAIL inhibited telomerase activity and the expression of telomerase catalytic subunit. CONCLUSION： TRAIL is an effective molecule to inhibit the growth of hepatocarcinoma through multiple pathways, such as inducing apoptosis and inhibiting the activity of telomerase.

关 键 词：细胞凋亡 配体 肝肿瘤 HEPG2细胞 

分 类 号：R363[医药卫生—病理学]